Hybrid scanning ion conductance and scanning near-field optical microscopy for the study of living cells

被引:61
作者
Korchev, YE
Raval, M
Lab, MJ
Gorelik, J
Edwards, CRW
Rayment, T
Klenerman, D
机构
[1] Univ London Imperial Coll Sci Technol & Med, MRC, Ctr Clin Sci, Div Med, London W12 0NN, England
[2] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England
[3] Univ London Imperial Coll Sci Technol & Med, Div Heart & Lung Inst, London W6 8RP, England
关键词
D O I
10.1016/S0006-3495(00)76811-1
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
We have developed a hybrid scanning ion conductance and scanning near-field optical microscope for the study of living cells. The technique allows quantitative, high-resolution characterization of the cell surface and the simultaneous recording of topographic and optical images. A particular feature of the method is a reliable mechanism to control the distance between the probe and the sample in physiological buffer. We demonstrate this new method by recording near-field images of living cells (cardiac myocytes).
引用
收藏
页码:2675 / 2679
页数:5
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