Optical chemical imaging of tobacco mosaic virus in solution at 60-nm resolution

被引:12
作者
Keller, TH [1 ]
Rayment, T [1 ]
Klenerman, D [1 ]
机构
[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England
基金
英国工程与自然科学研究理事会;
关键词
D O I
10.1016/S0006-3495(98)77914-7
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Scanning near-field optical microscopy can provide images with a resolution less than the wavelength of light, and therefore ought in principle to be of great value in studies of biological structures. In this work we show how for the first time images have been obtained of tobacco mosaic virus particles at 60-nm resolution, combined with chemical imaging using monoclonal antibodies under in vitro conditions.
引用
收藏
页码:2076 / 2079
页数:4
相关论文
共 11 条
[1]   Analysis of surface forces on oxides in aqueous solutions using AFM [J].
Arai, T ;
Aoki, D ;
Okabe, Y ;
Fujihira, M .
THIN SOLID FILMS, 1996, 273 (1-2) :322-326
[2]   VISUALIZATION BY ELECTRON-MICROSCOPY OF THE LOCATION OF TOBACCO MOSAIC-VIRUS EPITOPES REACTING WITH MONOCLONAL-ANTIBODIES IN ENZYME-IMMUNOASSAY [J].
DORE, I ;
WEISS, E ;
ALTSCHUH, D ;
VANREGENMORTEL, MHV .
VIROLOGY, 1988, 162 (02) :279-289
[3]   NEAR-FIELD OPTICAL-SCANNING MICROSCOPY [J].
DURIG, U ;
POHL, DW ;
ROHNER, F .
JOURNAL OF APPLIED PHYSICS, 1986, 59 (10) :3318-3327
[4]   Scanning near-field optical microscopy in reflection mode imaging in liquid [J].
Keller, TH ;
Rayment, T ;
Klenerman, D ;
Stephenson, RJ .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1997, 68 (03) :1448-1454
[5]  
Moyer PJ, 1996, APPL PHYS LETT, V68, P3380, DOI 10.1063/1.116510
[6]   NEAR-FIELD OPTICAL MICROSCOPY IN LIQUIDS [J].
MURAMATSU, H ;
CHIBA, N ;
HOMMA, K ;
NAKAJIMA, K ;
ATAKA, T ;
OHTA, S ;
KUSUMI, A ;
FUJIHIRA, M .
APPLIED PHYSICS LETTERS, 1995, 66 (24) :3245-3247
[7]   STRUCTURE OF TOBACCO MOSAIC-VIRUS AT 3.6-A RESOLUTION - IMPLICATIONS FOR ASSEMBLY [J].
NAMBA, K ;
STUBBS, G .
SCIENCE, 1986, 231 (4744) :1401-1406
[8]  
POHL DW, 1993, NATO ASI E, V242
[9]   VISCOELASTICITY OF LIVING CELLS ALLOWS HIGH-RESOLUTION IMAGING BY TAPPING MODE ATOMIC-FORCE MICROSCOPY [J].
PUTMAN, CAJ ;
VANDERWERF, KO ;
DEGROOTH, BG ;
VANHULST, NF ;
GREVE, J .
BIOPHYSICAL JOURNAL, 1994, 67 (04) :1749-1753
[10]   KINETIC AND FUNCTIONAL MAPPING OF VIRAL EPITOPES USING BIOSENSOR TECHNOLOGY [J].
SAUNAL, H ;
VANREGENMORTEL, MHV .
VIROLOGY, 1995, 213 (02) :462-471