Monitoring DPA release from a single germinating Bacillus subtilis endospore via surface-enhanced Raman scattering microscopy

被引：56

作者：

Evanoff, David D., Jr. ^{[1
]}

Heckel, John ^{[1
]}

Caldwell, Thomas P. ^{[1
]}

Christensen, Kenneth A. ^{[1
]}

Chumanov, George ^{[1
]}

机构：

[1] Clemson Univ, Dept Chem, Clemson, SC 29634 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 39期

关键词：

D O I：

10.1021/ja0642717

中图分类号：

O6 [化学];

学科分类号：

0703 [化学];

摘要：

A method for monitoring DPA release from a single germinating Bacillus subtilis endospore is reported. High S/N ratio SERS spectra were obtained with excitation power 3 mW at 647.1 nm and 1 min spectral collection times. The method is proof-of-principle for the SERS detection limit at the single spore level. This represents a 100- to 1000-fold improvement over previously reported detection limits for SERS-based measurements of DPA in endospores. Copyright © 2006 American Chemical Society.

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收藏

页码：12618 / 12619

页数：2

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