4D tracking of clinical seminal samples for quantitative characterization of motility parameters

被引：59

作者：

Di Caprio, Giuseppe ^{[1
]}

El Mallahi, Ahmed ^{[2
]}

Ferraro, Pietro ^{[3
]}

Dale, Roberta ^{[4
]}

Coppola, Gianfranco ^{[4
]}

Dale, Brian ^{[4
]}

Coppola, Giuseppe ^{[1
]}

Dubois, Frank ^{[2
]}

机构：

[1] Inst Microelect & Microsyst, Natl Res Council, Unit Naples, I-80121 Naples, Italy

[2] Univ Libre Bruxelles, Micrograv Res Ctr, B-1050 Brussels, Belgium

[3] Natl Inst Opt, Natl Res Council, Unit Naples, I-80078 Pozzuoli, Italy

[4] Ctr Assisted Fertilizat CFA, I-80123 Naples, Italy

来源：

BIOMEDICAL OPTICS EXPRESS | 2014年 / 5卷 / 03期

关键词：

DIGITAL HOLOGRAPHIC MICROSCOPY; PHASE-CONTRAST MICROSCOPY; PLANE DETECTION; RECONSTRUCTION; COMPENSATION; IMAGE;

D O I：

10.1364/BOE.5.000690

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper we investigate the use of a digital holographic microscope, with partial spatial coherent illumination, for the automated detection and tracking of spermatozoa. This in vitro technique for the analysis of quantitative parameters is useful for assessment of semen quality. In fact, thanks to the capabilities of digital holography, the developed algorithm allows us to resolve in-focus amplitude and phase maps of the cells under study, independently of focal plane of the sample image. We have characterized cell motility on clinical samples of seminal fluid. In particular, anomalous sperm cells were characterized and the quantitative motility parameters were compared to those of normal sperm. (C) 2014 Optical Society of America

引用

页码：690 / 700

页数：11

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