Imaging of mRNA in Live Cells Using Nucleic Acid-Templated Reduction of Azidorhodamine Probes

被引：131

作者：

Pianowski, Zbigniew ^{[1
]}

Gorska, Katarzyna ^{[1
]}

Oswald, Laurence ^{[1
]}

Merten, Christoph A. ^{[1
]}

Winssinger, Nicolas ^{[1
]}

机构：

[1] Univ Strasbourg, ISIS UMR 7006, CNRS, F-67000 Strasbourg, France

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 18期

基金：

欧洲研究理事会;

关键词：

QUENCHED AUTOLIGATION PROBES; LIVING CELLS; CATALYZED TRANSFER; MOLECULAR BEACONS; GENE-EXPRESSION; DNA; PEPTIDE; LIGATION; DISCRIMINATION; GLIOMAS;

D O I：

10.1021/ja809656k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nucleic acid-templated reactions leading to a fluorescent product represent an attractive strategy for the detection and imaging of cellular nucleic acids. Herein we report the use of a Staudinger reaction to promote the reduction of profluorescent azidorhodamine. The use of two cell-permeable GPNA probes, one labeled with the profluorescent azidorhodamine and the other with trialkylphosphine, enabled the detection of the mRNA encoding O-6-methylguanine-DNA methyltransferase in intact cells.

引用

页码：6492 / 6497

页数：6

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