Quinohemoprotein alcohol dehydrogenase-based reagentless amperometric biosensor for ethanol monitoring during wine fermentation

被引:75
作者
Niculescu, M
Erichsen, T
Sukharev, V
Kerenyi, Z
Csöregi, E
Schuhmann, W
机构
[1] Lund Univ, Dept Biotechnol, S-22100 Lund, Sweden
[2] Ruhr Univ Bochum, D-44780 Bochum, Germany
[3] Moscow MV Lomonosov State Univ, Dept Chem, RU-119899 Moscow, Russia
[4] Minist Agr, Res Inst Viticulture & Enol, H-6001 Kecskemet, Hungary
关键词
alcohol dehydrogenase; ethanol; biosensor; fermentation; on-line process control; redox polymer; sequential-injection analysis;
D O I
10.1016/S0003-2670(02)00344-6
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This paper describes the development and optimization of an amperometric biosensor for monitoring ethanol in beverages. The biosensor is constructed by cross-linking a quinoprotein alcohol dehydrogenase (QH-ADH) to an Os-complex-modified poly(vinylimidazole) redox polymer using poly(ethylene glycol) diglycidyl ether. The optimum biosensor configuration was evaluated by changing the ratio between enzyme, redox polymer, and cross-linker using conventional graphite rods as basis electrodes. The optimized sensor showed a sensitivity of 0.336+/-0.025 A M-1 cm(2) for ethanol and a detection limit (calculated as three times the signal-to-noise ratio) of 1 muM. This biosensor configuration was further evaluated in a conventional flow-injection system and the applicability for the determination of ethanol in diverse wine samples could be successfully demonstrated. Adaptation of this sensor configuration to screen-printed (SP) electrodes allowed their integration into an automated sequential-injection analyzer and the successful on-line monitoring of ethanol during wine fermentation processes. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:39 / 51
页数:13
相关论文
共 25 条
  • [11] KURZAWA C, 2001, GIT LABOR FACHZEITSC, V45, P156
  • [12] On-line monitoring of monosaccharides and ethanol during a fermentation by microdialysis sampling, liquid chromatography and two amperometric biosensors
    Liden, H
    Buttler, T
    Jeppsson, H
    Marko-Varga, G
    Volc, J
    Gorton, L
    [J]. CHROMATOGRAPHIA, 1998, 47 (9-10) : 501 - 508
  • [13] MARCINKEVICIENE L, 1999, BIOLOGIJA, V2, P109
  • [14] Ethanol amperometric biosensor based on an alcohol oxidase graphite-polymer biocomposite
    Morales, A
    Cespedes, F
    Martinez-Fabregas, E
    Alegret, S
    [J]. ELECTROCHIMICA ACTA, 1998, 43 (23) : 3575 - 3579
  • [15] AMPEROMETRIC DETERMINATION OF ALCOHOLS, ALDEHYDES AND CARBOXYLIC-ACIDS WITH AN IMMOBILIZED ALCOHOL OXIDASE ENZYME ELECTRODE
    NANJO, M
    GUILBAULT, GG
    [J]. ANALYTICA CHIMICA ACTA, 1975, 75 (01) : 169 - 180
  • [16] WIRED ENZYME ELECTRODES FOR AMPEROMETRIC DETERMINATION OF GLUCOSE OR LACTATE IN THE PRESENCE OF INTERFERING SUBSTANCES
    OHARA, TJ
    RAJAGOPALAN, R
    HELLER, A
    [J]. ANALYTICAL CHEMISTRY, 1994, 66 (15) : 2451 - 2457
  • [17] Determination of breath alcohol using a differential-type amperometric biosensor based on alcohol dehydrogenase
    Park, JK
    Yee, HJ
    Lee, KS
    Lee, WY
    Shin, MC
    Kim, TH
    Kim, SR
    [J]. ANALYTICA CHIMICA ACTA, 1999, 390 (1-3) : 83 - 91
  • [18] Polypyrrole entrapped quinohemoprotein alcohol dehydrogenase.: Evidence for direct electron transfer via conducting-polymer chains
    Ramanavicius, A
    Habermüller, K
    Csöregi, E
    Laurinavicius, V
    Schuhmann, W
    [J]. ANALYTICAL CHEMISTRY, 1999, 71 (16) : 3581 - 3586
  • [19] Razumiene J, 2002, ELECTROANAL, V14, P43, DOI 10.1002/1521-4109(200201)14:1<43::AID-ELAN43>3.0.CO
  • [20] 2-5