Invader plus method detects herpes simplex virus in cerebrospinal fluid and simultaneously differentiates types 1 and 2

被引：13

作者：

Allawi, Hatim T.

Li, Haijing

Sander, Tamara

Aslanukov, Azamat

Lyamichev, Victor I.

Blackman, Amondrea

Elagin, Slava

Tang, Yi-Wei

机构：

[1] Third Wave Technol Inc, Madison, WI 53719 USA

[2] Vanderbilt Univ, Med Ctr, Nashville, TN 37232 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 09期

关键词：

D O I：

10.1128/JCM.01175-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report here on the development and validation of a prototype Invader Plus method for the qualitative detection of herpes simplex virus types 1 and 2 in cerebrospinal fluid (CSF). The method combines PCR and Invader techniques in a single, closed-tube, continuous-reaction format that gives an analytical sensitivity (if approximately 10 copies per reaction. The clinical sensitivity and specificity were 100.0% and 98.6%, respectively, when the results of the method were validated against the results obtained with a PCR colorimetric microtiter plate system by use of clinical CSF specimens.

引用

页码：3443 / 3447

页数：5

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