Characterization of volatile organic compounds and odors by in-vivo sampling of beef cattle rumen gas, by solid-phase microextraction, and gas chromatography-mass spectrometry-olfactometry

被引:49
作者
Cai, Lingshuang
Koziel, Jacek A. [1 ]
Davis, Jeremiah
Lo, Yin-Cheung
Xin, Hongwei
机构
[1] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA 50011 USA
[2] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
关键词
rumen gas; odor; in-vivo sampling; SPME; GC-MS-O;
D O I
10.1007/s00216-006-0799-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Volatile organic compounds (VOCs) and odors in cattle rumen gas have been characterized by in-vivo headspace sampling by solid-phase microextraction (SPME) and analysis by gas chromatography-mass spectrometry-olfactometry (GC-MS-O). A novel device enabling headspace SPME (HS-SPME) sampling through a cannula was designed, refined, and used to collect rumen gas samples from steers. A Carboxen-polydimethylsiloxane (PDMS) fiber (85 mu m) was used for SPME sampling. Fifty VOCs from ten chemical groups were identified in the rumen headspace. The VOCs identified had a wide range of molecular weight (MW) (34 to 184), boiling point (-63.3 to 292 degrees C), vapor pressure (1.05 x 10(-5) to 1.17 x 10(2) Pa), and water solubility (0.66 to 1 x 10(6) mg L-1). Twenty-two of the compounds have a published odor detection thresholds (ODT) of less than 1 ppm. More than half of the compounds identified are reactive and have an estimated atmospheric lifetime of < 24 h. The amounts of VFAs, sulfide compounds, phenolic compounds, and skatole, and the odor intensity of VFAs and sulfide compounds in the rumen gas were all higher after feeding than before feeding. These results indicate that rumen gases can be an important potential source of aerial emissions of reactive VOCs and odor. In-vivo sampling by SPME then GC-MS-O analysis can be a useful tool for qualitative characterization of rumen gases, digestion, and its relationship to odor and VOC formation.
引用
收藏
页码:1791 / 1802
页数:12
相关论文
共 46 条
[1]   Relationship between fermentation acid production in the rumen and the requirement for physically effective fiber [J].
Allen, MS .
JOURNAL OF DAIRY SCIENCE, 1997, 80 (07) :1447-1462
[2]   Design and validation of portable SPME devices for rapid field air sampling and diffusion based calibration [J].
Augusto, F ;
Koziel, J ;
Pawliszyn, J .
ANALYTICAL CHEMISTRY, 2001, 73 (03) :481-486
[3]   EVIDENCE FOR THE CLIMATIC ROLE OF MARINE BIOGENIC SULFUR [J].
BATES, TS ;
CHARLSON, RJ ;
GAMMON, RH .
NATURE, 1987, 329 (6137) :319-321
[4]   GAS CHROMATOGRAPHIC ANALYSIS OF AMINES AND OTHER COMPOUNDS PRODUCED BY SEVERAL SPECIES OF CLOSTRIDIUM [J].
BROOKS, JB ;
MOORE, WEC .
CANADIAN JOURNAL OF MICROBIOLOGY, 1969, 15 (12) :1433-&
[5]   SIMULTANEOUS DETERMINATION OF LACTIC AND VOLATILE FATTY-ACIDS IN MICROBIAL FERMENTATION EXTRACTS BY GAS-LIQUID-CHROMATOGRAPHY [J].
BROTZ, PG ;
SCHAEFER, DM .
JOURNAL OF MICROBIOLOGICAL METHODS, 1987, 6 (03) :139-144
[6]   Characterization of volatile organic compounds and odorants associated with swine barn particulate matter using solid-phase microextraction and gas chromatography-mass spectrometry-olfactometry [J].
Cai, LS ;
Koziel, JA ;
Lo, YC ;
Hoff, SJ .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1102 (1-2) :60-72
[7]   Comparative analysis of gas production profiles obtained with buffalo and sheep ruminal fluid as the source of inoculum [J].
Calabrò, S ;
López, S ;
Piccolo, V ;
Dijkstra, J ;
Dhanoa, MS ;
France, J .
ANIMAL FEED SCIENCE AND TECHNOLOGY, 2005, 123 :51-65
[8]   OCEANIC PHYTOPLANKTON, ATMOSPHERIC SULFUR, CLOUD ALBEDO AND CLIMATE [J].
CHARLSON, RJ ;
LOVELOCK, JE ;
ANDREAE, MO ;
WARREN, SG .
NATURE, 1987, 326 (6114) :655-661
[9]  
CHURCH DC, 1988, RUMINANT ANIMAL
[10]  
Davis W.T., 2000, AIR POLLUTION ENG MA, VSecond