Luminescence-based colorimetric discrimination of single-nucleotide transversions by the combined use of the derivatives of DOTA-conjugated naphthyridine and its terbium complex

被引：13

作者：

Atsumi, Hiroshi ^{[1
]}

Yoshimoto, Keitaro ^{[1
,2
,3
,4
]}

Saito, Shingo ^{[3
,5
]}

Ohkuma, Moriya ^{[3
]}

Maeda, Mizuo ^{[3
]}

Nagasaki, Yukio ^{[1
,2
,4
,6
,7
]}

机构：

[1] Univ Tsukuba, Grad Sch Pure & Appl Sci, Tsukuba, Ibaraki 3058573, Japan

[2] Univ Tsukuba, Ctr Tsukuba Adv Res Alliance, Tsukuba, Ibaraki 3058577, Japan

[3] RIKEN, Bioengn Lab, Wako, Saitama 3510198, Japan

[4] Univ Tsukuba, Tsukuba Res Ctr Interdisciplinary Mat Sci, Tsukuba, Ibaraki 3058573, Japan

[5] Saitama Univ, Grad Sch Sci & Engn, Sakura Ku, Saitama 3388570, Japan

[6] Univ Tsukuba, Grad Sch Comprehens Human Sci, Masters Sch Med Sci, Tsukuba, Ibaraki 3058573, Japan

[7] NIMS, Int Ctr Mat Nanoarchilecton, Satellite Lab, Tsukuba, Ibaraki 3058573, Japan

来源：

TETRAHEDRON LETTERS | 2009年 / 50卷 / 19期

关键词：

Nucleobase; Fluorescence; Lanthanide energy transfer; Point mutation; DOTA; Terbium; GUANINE-GUANINE MISMATCHES; SITE-CONTAINING DNA; PYRIMIDINE/PURINE TRANSVERSION; FLUORESCENCE DETECTION; ENERGY-TRANSFER; TB3+ COMPLEX; BINDING; RECOGNITION; SEQUENCE; PROBES;

D O I：

10.1016/j.tetlet.2009.02.152

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We newly synthesized a nucleobase-binding ligand, ND-DOTA, in which 2-amino-5,7-dimethyl-1,8-naphthyridine (ND) was conjugated with 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (DOTA) via an amide linker, and found that its terbium(III) complex (ND-DOTA-Tb) showed green emission based on an energy transfer from the naphthyridine moiety to Tb3+. The blue emission of ND-DOTA was selectively quenched by adding abasic site-containing DNA duplexes that have pyrimidine bases opposite to the abasic site. In contrast, at the same excitation wavelength, ND-DOTA-Tb showed green emission independently of the bases opposite to the abasic site. Thus, a mixed solution of ND-DOTA and ND-DOTA-Tb enabled the luminescence-based colorimetric discrimination of single-nucleotide transversions with the naked eye at a single excitation wavelength. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：2177 / 2180

页数：4

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