Identification of Multiple Constituents in the TCM-Formula Shexiang Baoxin Pill by LC Coupled with DAD-ESI-MS-MS

被引：24

作者：

Jiang, Peng ^{[1
]}

Dou, Shengshan ^{[1
]}

Liu, Lei ^{[1
]}

Zhang, Weidong ^{[1
,2
]}

Chen, Zhongliang ^{[3
]}

Xu, Ruilin ^{[3
]}

Ding, Jianmi ^{[3
]}

Liu, Ruihui ^{[1
]}

机构：

[1] Second Mil Med Univ, Sch Pharm, Shanghai, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200030, Peoples R China

[3] Shanghai Hutchison Pharmaceut Co, Shanghai 200331, Peoples R China

来源：

CHROMATOGRAPHIA | 2009年 / 70卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Column liquid chromatography; Tandem mass spectrometry; Shexiang Baoxin Pill; PERFORMANCE LIQUID-CHROMATOGRAPHY; MAJOR CONSTITUENTS; CHINESE; MEDICINE;

D O I：

10.1365/s10337-009-1142-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An LC-DAD-ESI-MS(2) method was developed for simultaneous analysis of major chemical constituents in Shexiang Baoxin Pill (SBP), an important multiherbal formula in traditional Chinese medicine widely used for treating cardiovascular diseases. The chromatographic separation was performed on a Symmetry C-18 column (5 mu m, 250 x 4.6 mm), whilst water with 0.5% formic acid and acetonitrile were used as mobile phase. On the basis of the characteristic UV absorption profile, the information of molecular weight and structure provided by ESI-MS(2), 57 constituents from moschus, radix rhizoma ginseng, calculus bovis, cortex cinnamomi, styrax and venenum bufonis, were detected and 47 of them were identified in this study. The method can be the basis for the quality control of SBP.

引用

页码：133 / 142

页数：10

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