Random insertion of a TetR-Inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression

被引：11

作者：

Schlicht, Maximilian ^{[1
]}

Berens, Christian ^{[1
]}

Daam, Janko ^{[1
]}

Hillen, Wolfgang ^{[1
]}

机构：

[1] Univ Erlangen Nurnberg, Lehrstuhl Mikrobiol, D-91058 Erlangen, Germany

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 08期

关键词：

D O I：

10.1128/AEM.00739-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The insertion element InsTip alpha was constructed to generate protein expression data. It randomly fuses the TetR-inducing peptide Tip to the affected reading frame. Fusion protein expression is quantified by Tet-regulated reporter gene expression. The expression patterns of tagged Escherichia coli genes fully agree with published data from transcriptional fusions or microarrays, validating the Tip tag approach.

引用

页码：5637 / 5642

页数：6

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