Performance characteristics for flow injection immunoassay using monoclonal antibodies against s-triazine and 2,4-D herbicides

被引：16

作者：

Fránek, M ^{[1
]}

Deng, AP ^{[1
]}

Kolár, V ^{[1
]}

机构：

[1] Vet Res Inst, CS-62132 Brno, Czech Republic

来源：

ANALYTICA CHIMICA ACTA | 2000年 / 412卷 / 1-2期

关键词：

flow injection immunoassay; monoclonal antibodies; immunoreactor; regeneration; calibration;

D O I：

10.1016/S0003-2670(00)00744-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sequential injection instrument (ALITEA, USA) with a photometric and fluorometric detection unit S2000 (Ocean Optics) was employed for the development of flow injection immunoanalysis (FIIA). The monoclonal antibodies against atrazine, simazine and 2,4-D were immobilized on aminopropyl glass particles by means of avidin/biotin system and packed in plexiglass column of 18 mu l volume. Assay characteristics for individual antibody-reactors and regeneration effectivities for acid and alkaline solutions are described. An attempt to prepare a functional mixed antibody-reactor has not achieved success since regeneration conditions found for individual reactors were not compatible with one performance protocol. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：19 / 27

页数：9

共 24 条

[21] Construction and characterization of the direct piezoelectric immunosensor for atrazine operating in solution [J].