A high-throughput screening assay for hydroxynitrile lyase activity

被引：18

作者：

Andexer, Jennifer ^{[1
]}

Guterl, Jan-Karl ^{[1
]}

Pohl, Martina ^{[1
]}

Eggert, Thorsten ^{[1
]}

机构：

[1] Univ Dusseldorf, KFA Julich, Forschungszentrum, Inst Mol Enzyme Technol, D-52426 Julich, Germany

来源：

CHEMICAL COMMUNICATIONS | 2006年 / 40期

关键词：

D O I：

10.1039/b607863j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A high-throughput screening assay for hydroxynitrile lyase activity accepting a wide range of HNL-substrates is presented, which is useful either for enzyme fingerprinting or screening of huge variant libraries generated in metagenome or directed evolution approaches.

引用

页码：4201 / 4203

页数：3