A high-throughput screening assay for hydroxynitrile lyase activity

被引：18

作者：

Andexer, Jennifer ^{[1
]}

Guterl, Jan-Karl ^{[1
]}

Pohl, Martina ^{[1
]}

Eggert, Thorsten ^{[1
]}

机构：

[1] Univ Dusseldorf, KFA Julich, Forschungszentrum, Inst Mol Enzyme Technol, D-52426 Julich, Germany

来源：

CHEMICAL COMMUNICATIONS | 2006年 / 40期

关键词：

D O I：

10.1039/b607863j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A high-throughput screening assay for hydroxynitrile lyase activity accepting a wide range of HNL-substrates is presented, which is useful either for enzyme fingerprinting or screening of huge variant libraries generated in metagenome or directed evolution approaches.

引用

页码：4201 / 4203

页数：3

共 32 条

[11] ESTIMATION OF MICROQUANTITIES OF CYANIDE [J].

EPSTEIN, J .

ANALYTICAL CHEMISTRY, 1947, 19 (04) :272-274

[12]

Essers AJA, 1994, ACTA HORTIC, V375, P97, DOI DOI 10.17660/ACTAH0RTIC.1994.375.7

[13] The first recombinant hydroxynitrile lyase and its application in the synthesis of (S)-cyanohydrins [J].

Forster, S ;

Roos, J ;

Effenberger, F ;

Wajant, H ;

Sprauer, A .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1996, 35 (04) :437-439

[14] Comprehensive step-by-step engineering of an (R)-hydroxynitrile lyase for large-scale asymmetric synthesis [J].

Glieder, A ;

Weis, R ;

Skranc, W ;

Poechlauer, P ;

Dreveny, I ;

Majer, S ;

Wubbolts, M ;

Schwab, H ;

Gruber, K .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (39) :4815-4818

[15] The synthesis of chiral cyanohydrins by oxynitrilases [J].

Griengl, H ;

Schwab, H ;

Fechter, M .

TRENDS IN BIOTECHNOLOGY, 2000, 18 (06) :252-256

[16]

Hasslacher M, 1997, PROTEINS, V27, P438

[17] Screening for hydroxynitrile lyases in plants [J].

Hickel, A ;

Heinrich, G ;

Schwab, H ;

Griengl, H .

BIOTECHNOLOGY TECHNIQUES, 1997, 11 (01) :55-58

[18]

Hughes J, 1997, BIOTECHNOL BIOENG, V53, P332, DOI 10.1002/(SICI)1097-0290(19970205)53:3&lt

[19]

332::AID-BIT12&gt

[20]

3.0.CO

← 1 2 3 4 →