Changes in actin dynamics are involved in salicylic acid signaling pathway

被引：21

作者：

Matouskova, Jindriska ^{[1
,4
]}

Janda, Martin ^{[1
]}

Fiser, Radovan ^{[2
]}

Sasek, Vladimir ^{[3
]}

Kocourkova, Daniela ^{[3
]}

Burketova, Lenka ^{[3
]}

Duskova, Jirina ^{[4
]}

Martinec, Jan ^{[3
]}

Valentova, Olga ^{[1
]}

机构：

[1] Prague Inst Chem Technol, Dept Biochem & Microbiol, CR-16628 Prague 6, Czech Republic

[2] Charles Univ Prague, Fac Sci, Prague 12800 2, Czech Republic

[3] Acad Sci Czech Republ, Inst Expt Bot, CR-16502 Prague 6, Lysolaje, Czech Republic

[4] Charles Univ Prague, Fac Pharm Hradec Kralove, Hradec Kralove 50005, Czech Republic

来源：

PLANT SCIENCE | 2014年 / 223卷

关键词：

Actin dynamics; Salicylic acid; PR genes; Phosphatidic acid; PHOSPHATIDIC-ACID; PHOSPHOLIPASE-D; TRANSCRIPTION FACTORS; DEFENSE-RESPONSES; BINDING PROTEINS; CAPPING PROTEIN; LATRUNCULIN-B; RAPID GROWTH; ARABIDOPSIS; CELLS;

D O I：

10.1016/j.plantsci.2014.03.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Changes in actin cytoskeleton dynamics are one of the crucial players in many physiological as well as non-physiological processes in plant cells. Positioning of actin filament arrays is necessary for successful establishment of primary lines of defense toward pathogen attack, depolymerization leads very often to the enhanced susceptibility to the invading pathogen. On the other hand it was also shown that the disruption of actin cytoskeleton leads to the induction of defense response leading to the expression of PATHOGENESIS RELATED proteins (PR). In this study we show that pharmacological actin depolymerization leads to the specific induction of genes in salicylic acid pathway but not that involved in jasmonic acid signaling. Life imaging of leafs of Arabidopsis thaliana with GFP-tagged fimbrin (GFP-fABD2) treated with 1 mM salicylic acid revealed rapid disruption of actin filaments resembling the pattern viewed after treatment with 200 nM latrunculin B. The effect of salicylic acid on actin filament fragmentation was prevented by exogenous addition of phosphatidic acid, which binds to the capping protein and thus promotes actin polymerization. The quantitative evaluation of actin filament dynamics is also presented. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

引用

页码：36 / 44

页数：9

共 42 条

[21] PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR [J].

Marshall, OJ .

BIOINFORMATICS, 2004, 20 (15) :2471-2472

[22]

Metraux J. P., 1991, Advances in molecular genetics of plant-microbe interactions. Vol. 1. Proceedings of the 5th international symposium on the molecular genetics of plant-microbe interactions, Interlaken, Switzerland, September 9-14, 1990., P432

[23] A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].

MURASHIGE, T ;

SKOOG, F .

PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497

[24] Hormonal Modulation of Plant Immunity [J].

Pieterse, Corne M. J. ;

Van der Does, Dieuwertje ;

Zamioudis, Christos ;

Leon-Reyes, Antonio ;

Van Wees, Saskia C. M. .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 28, 2012, 28 :489-521

[25] Regulation of cytoskeletal dynamics by phospholipase D and phosphatidic acid [J].

Pleskot, Roman ;

Li, Jiejie ;

Zarsky, Viktor ;

Potocky, Martin ;

Staiger, Christopher J. .

TRENDS IN PLANT SCIENCE, 2013, 18 (09) :496-504

[26] Mutual regulation of plant phospholipase D and the actin cytoskeleton [J].

Pleskot, Roman ;

Potocky, Martin ;

Pejchar, Premysl ;

Linek, Jan ;

Bezvoda, Radek ;

Martinec, Jan ;

Valentova, Olga ;

Novotna, Zuzana ;

Zarsky, Viktor .

PLANT JOURNAL, 2010, 62 (03) :494-507

[27] WRKY transcription factors [J].

Rushton, Paul J. ;

Somssich, Imre E. ;

Ringler, Patricia ;

Shen, Qingxi J. .

TRENDS IN PLANT SCIENCE, 2010, 15 (05) :247-258

[28] Plant pathogenesis-related (PR) proteins: A focus on PR peptides [J].

Sels, Jan ;

Mathys, Janick ;

De Coninck, Barbara M. A. ;

Cammue, Bruno P. A. ;

De Bolle, Miguel F. C. .

PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2008, 46 (11) :941-950

[29] Annealing accounts for the length of actin filaments formed by spontaneous polymerization [J].

Sept, D ;

Xu, JY ;

Pollard, TD ;

McCammon, JA .

BIOPHYSICAL JOURNAL, 1999, 77 (06) :2911-2919

[30] How do plants achieve immunity? Defence without specialized immune cells [J].

Spoel, Steven H. ;

Dong, Xinnian .

NATURE REVIEWS IMMUNOLOGY, 2012, 12 (02) :89-100

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