Multiple mitochondrial DNA polymerases in Trypanosoma brucei

被引：111

作者：

Klingbeil, MM ^{[1
]}

Motyka, SA ^{[1
]}

Englund, PT ^{[1
]}

机构：

[1] Johns Hopkins Med Sch, Dept Biol Chem, Baltimore, MD 21205 USA

来源：

MOLECULAR CELL | 2002年 / 10卷 / 01期

关键词：

D O I：

10.1016/S1097-2765(02)00571-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Kinetoplast DNA (kDNA), the unusual mitochondrial DNA of Trypanosoma brucei, is a network containing thousands of catenated circles. Database searching for a kDNA replicative polymerase (pol) revealed no mitochondrial pol gamma homolog. Instead, we identified four proteins (TbPOLIA, IB, IC, and ID) related to bacterial pol 1. Remarkably, all four localized to the mitochondrion. TbPOLIB and TbPOLIC localized beside the kDNA where replication occurs, and their knockdown by RNA interference caused kDNA network shrinkage. Furthermore, silencing of TbPOILIC caused loss of both minicircles and maxicircles and accumulation of minicircle replication intermediates, consistent with a role in replication. While typical mitochondria contain one DNA polymerase, pol gamma, trypanosome mitochondria contain five such enzymes, including the previously characterized pol beta.

引用

页码：175 / 186

页数：12

共 42 条

[31] Evolution of the protists and protistan parasites from the perspective of molecular systematics [J].

Sogin, ML ;

Silberman, JD .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1998, 28 (01) :11-20

[32]

Sousa R, 1996, TRENDS BIOCHEM SCI, V21, P186, DOI 10.1016/S0968-0004(96)10023-2

[33] Structural biology - A mechanism for all polymerases [J].

Steitz, TA .

NATURE, 1998, 391 (6664) :231-232

[34] Quartet puzzling: A quartet maximum-likelihood method for reconstructing tree topologies [J].

Strimmer, K ;

vonHaeseler, A .

MOLECULAR BIOLOGY AND EVOLUTION, 1996, 13 (07) :964-969

[35] A DNA-POLYMERASE-BETA IN THE MITOCHONDRION OF THE TRYPANOSOMATID CRITHIDIA-FASCICULATA [J].

TORRI, AF ;

ENGLUND, PT .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (08) :3495-3497

[36]

TORRI AF, 1994, J BIOL CHEM, V269, P8165

[37] The mus308 locus of Drosophila melanogaster is implicated in the bypass of ENU-induced O-alkylpyrimidine adducts [J].

Tosal, L ;

Comendador, MA ;

Sierra, LM .

MOLECULAR AND GENERAL GENETICS, 2000, 263 (01) :144-151

[38] UNIVERSAL MINICIRCLE SEQUENCE BINDING-PROTEIN, A CCHC-TYPE ZINC-FINGER PROTEIN THAT BINDS THE UNIVERSAL MINICIRCLE SEQUENCE OF TRYPANOSOMATIDS - PURIFICATION AND CHARACTERIZATION [J].

TZFATI, Y ;

ABELIOVICH, H ;

AVRAHAMI, D ;

SHLOMAI, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (36) :21339-21345

[39] ELECTROPORATION IN INTRACELLULAR BUFFER INCREASES CELL-SURVIVAL [J].

VANDENHOFF, MJB ;

MOORMAN, AFM ;

LAMERS, WH .

NUCLEIC ACIDS RESEARCH, 1992, 20 (11) :2902-2902

[40] RNA interference of a trypanosome topoisomerase II causes progressive loss of mitochondrial DNA [J].

Wang, ZF ;

Englund, PT .

EMBO JOURNAL, 2001, 20 (17) :4674-4683

← 1 2 3 4 5 →