Microscopic imaging of DNA repair foci in irradiated normal tissues

被引：46

作者：

Bhogal, Nirmal ^{[1
]}

Jalali, Farid ^{[1
]}

Bristow, Robert G. ^{[1
,2
,3
]}

机构：

[1] Princess Margaret Hosp, Ontario Canc Inst, UHN, Appl Mol Oncol & Radiat Med Program, Toronto, ON M5G 2M9, Canada

[2] Univ Toronto, Dept Med Biophys, Toronto, ON M5S 1A1, Canada

[3] Univ Toronto, Dept Radiat Oncol, Toronto, ON M5S 1A1, Canada

来源：

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 2009年 / 85卷 / 09期

关键词：

Biomarker; DNA repair; -H2AX; deconvolution; foci; confocal microscopy; normal tissues; ionising radiation; DOUBLE-STRAND BREAKS; FIELD GEL-ELECTROPHORESIS; PHOSPHORYLATED HISTONE H2AX; GAMMA-H2AX FOCI; IN-SITU; QUANTITATIVE-ANALYSIS; CONFOCAL MICROSCOPY; RESTRICTION ENZYMES; BLOOD-LYMPHOCYTES; TUMOR-SUPPRESSOR;

D O I：

10.1080/09553000902785791

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Purpose: It is now feasible to detect DNA double strand breaks (DSB) in tissues by measuring the induction and resolution of DNA repair foci, such as -H2AX, using immunofluorescent microscopy and digital image analysis. This review will highlight principal tools and approaches to tissue microscopy and analysis. It will also discuss the practical considerations of using microscopy invitro and invivo in measuring intranuclear foci following irradiation. Conclusions: Computer-based image analysis algorithms allow an objective and quantitative analysis of foci and protein-protein interactions using 3D confocal images. Finally, we review the literature in which DNA repair foci have been investigated as a biodosimeter or a biomarker of DNA repair in normal tissues.

引用

页码：732 / 746

页数：15

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