Microscopic imaging of DNA repair foci in irradiated normal tissues

被引：46

作者：

Bhogal, Nirmal ^{[1
]}

Jalali, Farid ^{[1
]}

Bristow, Robert G. ^{[1
,2
,3
]}

机构：

[1] Princess Margaret Hosp, Ontario Canc Inst, UHN, Appl Mol Oncol & Radiat Med Program, Toronto, ON M5G 2M9, Canada

[2] Univ Toronto, Dept Med Biophys, Toronto, ON M5S 1A1, Canada

[3] Univ Toronto, Dept Radiat Oncol, Toronto, ON M5S 1A1, Canada

来源：

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 2009年 / 85卷 / 09期

关键词：

Biomarker; DNA repair; -H2AX; deconvolution; foci; confocal microscopy; normal tissues; ionising radiation; DOUBLE-STRAND BREAKS; FIELD GEL-ELECTROPHORESIS; PHOSPHORYLATED HISTONE H2AX; GAMMA-H2AX FOCI; IN-SITU; QUANTITATIVE-ANALYSIS; CONFOCAL MICROSCOPY; RESTRICTION ENZYMES; BLOOD-LYMPHOCYTES; TUMOR-SUPPRESSOR;

D O I：

10.1080/09553000902785791

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Purpose: It is now feasible to detect DNA double strand breaks (DSB) in tissues by measuring the induction and resolution of DNA repair foci, such as -H2AX, using immunofluorescent microscopy and digital image analysis. This review will highlight principal tools and approaches to tissue microscopy and analysis. It will also discuss the practical considerations of using microscopy invitro and invivo in measuring intranuclear foci following irradiation. Conclusions: Computer-based image analysis algorithms allow an objective and quantitative analysis of foci and protein-protein interactions using 3D confocal images. Finally, we review the literature in which DNA repair foci have been investigated as a biodosimeter or a biomarker of DNA repair in normal tissues.

引用

页码：732 / 746

页数：15

共 81 条

[31] DNA damage responses: Mechanisms and roles in human disease [J].

Kastan, Michael B. .

MOLECULAR CANCER RESEARCH, 2008, 6 (04) :517-524

[32] γ-H2AX foci after low-dose-rate irradiation reveal Atm haploinsufficiency in mice [J].

Kato, Takamitsu A. ;

Nagasawa, Hatsumi ;

Weil, Michael M. ;

Genik, Paula C. ;

Little, J. B. ;

Bedford, J. S. .

RADIATION RESEARCH, 2006, 166 (01) :47-54

[33] Heterochromatin is refractory to γ-H2AX modification in yeast and mammals [J].

Kim, Jung-Ae ;

Kruhlak, Michael ;

Dotiwala, Farokh ;

Nussenzweig, Andre ;

Haber, James E. .

JOURNAL OF CELL BIOLOGY, 2007, 178 (02) :209-218

[34] γ-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin [J].

Kinner, Andrea ;

Wu, Wenqi ;

Staudt, Christian ;

Iliakis, George .

NUCLEIC ACIDS RESEARCH, 2008, 36 (17) :5678-5694

[35] Phosphorylated histone H2AX in relation to cell survival in tumor cells and xenografts exposed to single and fractionated doses of X-rays [J].

Klokov, Dmitry ;

MacPhail, Susan M. ;

Banath, Judit P. ;

Byrne, James P. ;

Olive, Peggy L. .

RADIOTHERAPY AND ONCOLOGY, 2006, 80 (02) :223-229

[36] Molecular mechanism of the recruitment of NBS1/hMRE11/hRAD50 complex to DNA double-strand breaks:: NBS1 binds to γ-H2AX through FHA/BRCT domain [J].

Kobayashi, J .

JOURNAL OF RADIATION RESEARCH, 2004, 45 (04) :473-478

[37] p53 phosphorylation in mouse skin and in vitro human skin model by high-dose-radiation exposure [J].

Koike, M ;

Sugasawa, J ;

Koike, A ;

Kohno, Y .

JOURNAL OF RADIATION RESEARCH, 2005, 46 (04) :461-468

[38] Histone H2AX phosphorylation independent of ATM after X-irradiation in mouse liver and kidney in situ [J].

Koike, Manabu ;

Mashino, Minako ;

Sugasawa, Jun ;

Koike, Aki .

JOURNAL OF RADIATION RESEARCH, 2008, 49 (04) :445-449

[39] Dynamic change of histone H2AX phosphorylation independent of ATM and DNA-PK in mouse skin in situ [J].

Koike, Manabu ;

Mashino, Minako ;

Sugasawa, Jun ;

Koike, Aki .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 363 (04) :1009-1012

[40] Induction and quantification of γ-H2AX foci following low and high LET-irradiation [J].

Leatherbarrow, EL ;

Harper, JV ;

Cucinotta, FA ;

O'Neill, P .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2006, 82 (02) :111-118

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