Antibacterial discovery in actinomycetes strains with mutations in RNA polymerase or ribosomal protein S12

被引：178

作者：

Hosaka, Takeshi ^{[2
]}

Ohnishi-Kameyama, Mayumi ^{[2
]}

Muramatsu, Hideyuki ^{[1
]}

Murakami, Kana ^{[1
]}

Tsurumi, Yasuhisa ^{[1
]}

Kodani, Shinya ^{[2
]}

Yoshida, Mitsuru ^{[2
]}

Fujie, Akihiko ^{[1
]}

Ochi, Kozo ^{[2
]}

机构：

[1] Astellas Pharma Inc, Fermentat Res Labs, Tsukuba, Ibaraki, Japan

[2] Natl Food Res Inst, Tsukuba, Ibaraki 305, Japan

来源：

NATURE BIOTECHNOLOGY | 2009年 / 27卷 / 05期

关键词：

COMPLETE GENOME SEQUENCE; STREPTOMYCES-COELICOLOR A3(2); ANTIBIOTIC PRODUCTION; ACTIVATION; RESISTANCE; LIVIDANS; MUTANT;

D O I：

10.1038/nbt.1538

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We show that selection of drug-resistant bacterial mutants allows the discovery of antibacterial compounds. Mutant strains of a soil-isolated Streptomyces species that does not produce antibacterials synthesize a previously unknown class of antibacterial, which we name piperidamycin. Overall, 6% of non-Streptomyces actinomycetes species and 43% of Streptomyces species that do not produce antibacterials are activated to produce them. The antibacterial-producing mutants all carried mutations in RNA polymerase and/or the ribosomal protein S12.

引用

页码：462 / 464

页数：3

共 16 条

[1] Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2) [J].

Bentley, SD ;

Chater, KF ;

Cerdeño-Tárraga, AM ;

Challis, GL ;

Thomson, NR ;

James, KD ;

Harris, DE ;

Quail, MA ;

Kieser, H ;

Harper, D ;

Bateman, A ;

Brown, S ;

Chandra, G ;

Chen, CW ;

Collins, M ;

Cronin, A ;

Fraser, A ;

Goble, A ;

Hidalgo, J ;

Hornsby, T ;

Howarth, S ;

Huang, CH ;

Kieser, T ;

Larke, L ;

Murphy, L ;

Oliver, K ;

O'Neil, S ;

Rabbinowitsch, E ;

Rajandream, MA ;

Rutherford, K ;

Rutter, S ;

Seeger, K ;

Saunders, D ;

Sharp, S ;

Squares, R ;

Squares, S ;

Taylor, K ;

Warren, T ;

Wietzorrek, A ;

Woodward, J ;

Barrell, BG ;

Parkhill, J ;

Hopwood, DA .

NATURE, 2002, 417 (6885) :141-147

[2]

Bode HB, 2002, CHEMBIOCHEM, V3, P619, DOI 10.1002/1439-7633(20020703)3:7<619::AID-CBIC619>3.0.CO

[3]

2-9

[4] The impact of bacterial genomics on natural product research [J].

Bode, HB ;

Müller, R .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (42) :6828-6846

[5] New antibiotics from bacterial natural products [J].

Clardy, Jon ;

Fischbach, Michael A. ;

Walsh, Christopher T. .

NATURE BIOTECHNOLOGY, 2006, 24 (12) :1541-1550

[6] Increased expression of ribosome recycling factor is responsible for the enhanced protein synthesis during the late growth phase in an antibiotic-overproducing Streptomyces coelicolor ribosomal rpsL mutant [J].

Hosaka, Takeshi ;

Xu, Jun ;

Ochi, Kozo .

MOLECULAR MICROBIOLOGY, 2006, 61 (04) :883-897

[7] Activation of antibiotic biosynthesis by specified mutations in the rpoB gene (encoding the RNA polymerase β subunit) of Streptomyces lividans [J].

Hu, HF ;

Zhang, Q ;

Ochi, K .

JOURNAL OF BACTERIOLOGY, 2002, 184 (14) :3984-3991

[8] Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis [J].

Ikeda, H ;

Ishikawa, J ;

Hanamoto, A ;

Shinose, M ;

Kikuchi, H ;

Shiba, T ;

Sakaki, Y ;

Hattori, M ;

Omura, S .

NATURE BIOTECHNOLOGY, 2003, 21 (05) :526-531

[9]

Ochi K, 2004, ADV APPL MICROBIOL, V56, P155, DOI 10.1016/s0065-2164(04)56005-7

[10] Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350 [J].

Ohnishi, Yasuo ;

Ishikawa, Jun ;

Hara, Hirofumi ;

Suzuki, Hirokazu ;

Ikenoya, Miwa ;

Ikeda, Haruo ;

Yamashita, Atsushi ;

Hattori, Masahira ;

Horinouchi, Sueharu .

JOURNAL OF BACTERIOLOGY, 2008, 190 (11) :4050-4060

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