Antibacterial discovery in actinomycetes strains with mutations in RNA polymerase or ribosomal protein S12

被引：178

作者：

Hosaka, Takeshi ^{[2
]}

Ohnishi-Kameyama, Mayumi ^{[2
]}

Muramatsu, Hideyuki ^{[1
]}

Murakami, Kana ^{[1
]}

Tsurumi, Yasuhisa ^{[1
]}

Kodani, Shinya ^{[2
]}

Yoshida, Mitsuru ^{[2
]}

Fujie, Akihiko ^{[1
]}

Ochi, Kozo ^{[2
]}

机构：

[1] Astellas Pharma Inc, Fermentat Res Labs, Tsukuba, Ibaraki, Japan

[2] Natl Food Res Inst, Tsukuba, Ibaraki 305, Japan

来源：

NATURE BIOTECHNOLOGY | 2009年 / 27卷 / 05期

关键词：

COMPLETE GENOME SEQUENCE; STREPTOMYCES-COELICOLOR A3(2); ANTIBIOTIC PRODUCTION; ACTIVATION; RESISTANCE; LIVIDANS; MUTANT;

D O I：

10.1038/nbt.1538

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We show that selection of drug-resistant bacterial mutants allows the discovery of antibacterial compounds. Mutant strains of a soil-isolated Streptomyces species that does not produce antibacterials synthesize a previously unknown class of antibacterial, which we name piperidamycin. Overall, 6% of non-Streptomyces actinomycetes species and 43% of Streptomyces species that do not produce antibacterials are activated to produce them. The antibacterial-producing mutants all carried mutations in RNA polymerase and/or the ribosomal protein S12.

引用

页码：462 / 464

页数：3

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