Inhibition of the alternative oxidase stimulates H2O2 production in plant mitochondria

被引：147

作者：

Popov, VN

Simonian, RA

Skulachev, VP

Starkov, AA

机构：

[1] MOSCOW MV LOMONOSOV STATE UNIV,AN BELOZERSKY INST PHYS CHEM BIOL,DEPT BIOENERGET,MOSCOW 119899,RUSSIA

[2] VORONEZH STATE UNIV,DEPT BIOL,VORONEZH 394693,RUSSIA

来源：

FEBS LETTERS | 1997年 / 415卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

alternative oxidase; antioxygen defence; reactive oxygen species; H2O2; plant mitochondrion;

D O I：

10.1016/S0014-5793(97)01099-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hypothesis that a non-coupled alternative oxidase of plant mitochondria operates as an antioxygen defence mechanism [Purvis, A.C. and Shewfelt, R.L., Physiol, Plant, 88 (1993) 712-718; Skulachev, V.P., Biochemistry (Moscow) 59 (1994) 1433-1434] has been confirmed in experiments on isolated soybean and pea cotyledon mitochondria, It is shown that inhibitors of the alternative oxidase, salicyl hydroxamate and propyl gallate strongly stimulate H2O2 production by these mitochondria oxidizing succinate. Effective concentrations of the inhibitors proved to be the same as those decreasing the cyanide-resistant respiration, The inhibitors proved to be ineffective in stimulating H2O2 formation in rat liver mitochondria lacking the alternative oxidase, (C) 1997 Federation of European Biochemical Societies.

引用

页码：87 / 90

页数：4

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