Impact and progress in small and wide angle X-ray scattering (SAXS and WAXS)

被引:157
作者
Graewert, Melissa A. [1 ]
Svergun, Dmitri I. [1 ]
机构
[1] DESY, EMBL, Hamburg Unit, European Mol Biol Lab, D-22603 Hamburg, Germany
关键词
INTRINSICALLY DISORDERED PROTEINS; LOW-RESOLUTION STRUCTURE; BIOLOGICAL MACROMOLECULES; GLOSSOSCOLEX-PAULISTUS; STRUCTURAL DIVERSITY; HEMOGLOBIN; COMPUTATION; PROFILES; ENSEMBLE; MODEL;
D O I
10.1016/j.sbi.2013.06.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The advances made in small and wide angle X-ray scattering over the past decades have had a large impact on structural biology. Many new insights into challenging biological probes including large and transient complexes, flexible macromolecules as well as other exciting objects of various sizes were gained with this low resolution technique. Here, we review the recent developments in the experimental setups and in software for data collection and analysis, specifically for hybrid approaches. These progresses have allowed scientists to address a number of intriguing questions which could not be answered with other structural methods alone.
引用
收藏
页码:748 / 754
页数:7
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