Quantitative analysis of colistin A and colistin B in plasma and culture medium using a simple precipitation step followed by LC/MS/MS

被引：95

作者：

Jansson, Britt ^{[1
]}

Karvanen, Matti ^{[2
]}

Cars, Otto ^{[2
]}

Plachouras, Diamantis ^{[3
]}

Friberg, Lena E. ^{[1
]}

机构：

[1] Biomedicum, Dept Biopharmaceut Sci, Div Pharmacokinet & Drug Therapy, SE-75124 Uppsala, Sweden

[2] Univ Uppsala Hosp, Dept Med Sci, Infect Dis Sect, SE-75185 Uppsala, Sweden

[3] Univ Athens, Dept Internal Med 4, Sch Med, Univ Hosp ATTICON, GR-12462 Haidari, Greece

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2009年 / 49卷 / 03期

关键词：

Colistin; CMS; LC/MS/MS; Plasma stability; Culture medium; PERFORMANCE LIQUID-CHROMATOGRAPHY; METHANESULFONATE; STABILITY; URINE; ASSAY;

D O I：

10.1016/j.jpba.2008.12.016

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An analytical method for quantitation of colistin A and colistin B in plasma and culture medium is described. After protein precipitation with acetonitrile (ACN) containing 0.1% trifluoroacetic acid (TFA), the supernatants were diluted with 0.03% TFA. The compounds were separated on an Ultrasphere C18 column, 4.6 mm x 250 mm, 5 mu m particle size with a mobile phase consisting of 25% ACN in 0.03% TFA and detected with tandem mass spectrometry. The instrument was operating in ESI negative ion mode and the precursor-product ion pairs were m/z 1167.7 -> 1079.6 for colistin A and m/z 1153.7 -> 1065.6 for colistin B. The lower limit of quantification (LLOQ) for 100 mu L plasma was 19.4 and 10.5 ng/mL for colistin A and B, respectively, with CV <6.2% and accuracy <+/- 12.6%. For culture medium (50 mu L+ 50 mu L plasma), LLOQ was 24.2 and 13.2 ng/mL for colistin A and B, respectively, with CV <11.4% and accuracy <+/- 8.1%. The quick sample work-up method allows for determination of colistin A and B in clinical samples without causing hydrolysis of the prodrug colistin methanesulfonate (CMS). (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：760 / 767

页数：8