Generation of large numbers of dendritic cells in a closed system using Cell Factories™

被引:86
作者
Tuyaerts, S
Noppe, SM
Corthals, J
Breckpot, K
Heirman, C
De Greef, C
Van Riet, I
Thielemans, K
机构
[1] Free Univ Brussels, Sch Med, Dept Physiol Immunol, Lab Mol & Cellular Therapy, B-1090 Brussels, Belgium
[2] Free Univ Brussels, Akad Ziekenhuis, Stem Cell Lab, B-1090 Brussels, Belgium
关键词
dendritic cells; clinical-grade cellular vaccine; immunotherapy; Cell Factory (TM);
D O I
10.1016/S0022-1759(02)00099-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
There is a growing interest in using dendritic cells (DC) for vaccine approaches in the treatment of cancer and infectious diseases. This requires a reproducible method for the generation of large numbers of DC in a closed culture system suitable for clinical use and conforming to the current guidelines of good manufacturing practices. We designed a system in which the DC were generated in a closed system from adherent monocytes using Cell Factories(TM) (DC-CF). Monocytes were enriched from apheresis products by adherence and then cultured in the presence of AB serum or autologous plasma and GM-CSF and IL-4 for 6 days. The DC generated in Cell Factories were extensively compared to research-grade DC generated in conventional tissue culture flasks (DC-TCF). At day 6, the immature DC were harvested and the yield, the viability, the immunophenotype and the functional characteristics of the DC were compared, DC-CF and DC-TCF showed similar viability and purity and scored equally when tested for stability, dextran and latex bead uptake, in MLR and in the activation of influenza-specific memory cells after electroporation with influenza matrix protein 1 (IMP1) mRNA. These data indicated that large numbers of functional clinical-grade DC could be generated from adherent cells in a closed system using Cell Factories(TM). (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:135 / 151
页数:17
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