Trypanosoma cruzi: The major cysteinyl proteinase (cruzipain) is a relevant immunogen of parasite acidic antigens (FIII)

被引：17

作者：

Laderach, D ^{[1
]}

Cerban, F ^{[1
]}

Motran, C ^{[1
]}

DeCima, EV ^{[1
]}

Gea, S ^{[1
]}

机构：

[1] UNIV NACL CORDOBA, FAC CIENCIAS QUIM, DEPT BIOQUIM CLIN, RA-5016 CORDOBA, ARGENTINA

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1996年 / 26卷 / 11期

关键词：

Trypanosoma cruzi; cruzipain; immune response; acidic antigens;

D O I：

10.1016/S0020-7519(96)00099-9

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

This study examined the immune responses induced by cruzipain, a web-characterized T. cruzi antigen, to determine whether it is a relevant immunogen among the parasite acidic antigens (FIII), for which some biological properties have been studied previously. Humoral and cellular immune responses were investigated in BALB/C mice after immunization with cruzipain or FIII. Skin tests revealed immediate type-hypersensitivity (ITH) and delayed-type hypersensitivity (DTH) reactions to cruzipain in both groups of immunized mice. IgG1 and IgE isotypes against cruzipain were detected by ELISA in both groups and immunoblot studies showed that these antibodies recognized a major protein band of 50 kDa, cruzipain. The antigen-specific proliferative responses of spleen lymphocytes from both groups of immunized mice were also increased. Immunization with cruzipain or Fm antigen significantly enhanced the percentage survival of mice challenged with 10(3) trypomastigotes. The results revealed high cross-reactivity between cruzipain and FIII, suggesting that cruzipain is a relevant immunogen among the parasite acidic antigens. Copyright (C) 1996 Australian Society for Parasitology. Published by Elsevier Science Ltd.

引用

页码：1249 / 1254

页数：6

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