Automated four-color DNA sequencing using primers assembled by hexamer ligation

被引：6

作者：

Kaczorowski, T

Szybalski, W

机构：

[1] UNIV WISCONSIN,MCARDLE LAB CANC RES,SCH MED,MADISON,WI 53706

[2] UNIV GDANSK,DEPT MICROBIOL,PL-80822 GDANSK,POLAND

来源：

GENE | 1996年 / 179卷 / 01期

关键词：

SPEL-6; T4 DNA ligase; DNA sequencer; four-color fluorescent terminators;

D O I：

10.1016/S0378-1119(96)00325-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A procedure based on the assembly of sequencing primers by hexamer ligation and then using them in automated DNA sequencing is described. This method is based on a four-color fluorescent terminator chemistry. Sequencing ladders were analyzed using an ABI 373 DNA sequencer (Applied Biosystems, Foster City, CA, USA). The best results were obtained for primers assembled by ligation of four to ten hexamers. The accuracy of the method was estimated to be 99.5% up to 400 nt of the read sequence, and somewhat lower at 400-600 nt.

引用

收藏

页码：195 / 198

页数：4

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