Chromosomal location of AFLP markers in common wheat utilizing nulli-tetrasomic stocks

被引:60
作者
Huang, XQ [1 ]
Zeller, FJ [1 ]
Hsam, SLK [1 ]
Wenzel, G [1 ]
Mohler, V [1 ]
机构
[1] Tech Univ Munich, Lehrstuhl Pflanzenbau & Pflanzenzuchtung, D-85350 Freising Weihenstephan, Germany
关键词
aneuploid; chromosome assignment; Triticum aestivum;
D O I
10.1139/gen-43-2-298
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Amplified fragment length polymorphism (AFLP) markers with a total of 256 EcoRI + ANN- MseI + CNN primer combinations were investigated employing the common wheat cultivar Triticum aestivum 'Chinese Spring.' On average, 103 fragments per primer combination were amplified, ranging from a maximum of 226 fragments to a minimum of 18 fragments. The primer combinations E + AAA - M + CNN and E + ATT - M + CNN produced very few distinct fragments. By using 15 randomly chosen EcoRI + ANN - MseI + CNN primer combinations, 928 AFLP markers were allocated to wheat chromosomes, of which 131 were assigned to specific chromosome arms. These AFLP markers were locus-specific and randomly distributed on the different chromosomes. In addition, 6 and 41 AFLP markers were simultaneously absent in two nulli-tetrasomics (NTs) of both homoeologous and non-homoeologous groups, respectively, whereas additional fragments were detected in N1BT1A, N5AT5D, and N6BT6A lines.
引用
收藏
页码:298 / 305
页数:8
相关论文
共 34 条
[1]   Development of a chromosomal arm map for wheat based on RFLP markers [J].
Anderson, J. A. ;
Ogihara, Y. ;
Sorrells, M. E. ;
Tanksley, S. D. .
THEORETICAL AND APPLIED GENETICS, 1992, 83 (08) :1035-1043
[2]  
BAIG, 1999, PHYTOPATHOLOGY, V89, P343
[3]   AFLP-based genetic diversity assessment among wheat cultivars from the Pacific Northwest [J].
Barrett, BA ;
Kidwell, KK .
CROP SCIENCE, 1998, 38 (05) :1261-1271
[4]   Predicting progeny variance from parental divergence in hard red spring wheat [J].
Burkhamer, RL ;
Lanning, SP ;
Martens, RJ ;
Martin, JM ;
Talbert, LE .
CROP SCIENCE, 1998, 38 (01) :243-248
[5]   RFLP-BASED GENETIC MAPS OF WHEAT HOMOLOGOUS GROUP-7 CHROMOSOMES [J].
CHAO, S ;
SHARP, PJ ;
WORLAND, AJ ;
WARHAM, EJ ;
KOEBNER, RMD ;
GALE, MD .
THEORETICAL AND APPLIED GENETICS, 1989, 78 (04) :495-504
[6]   CHROMOSOMAL LOCALIZATION OF INTERGENOMIC RFLP LOCI IN HEXAPLOID WHEAT [J].
DEVEY, ME ;
HART, GE .
GENOME, 1993, 36 (05) :913-918
[7]   Identification of AFLP markers closely linked to the powdery mildew resistance genes Pm1c and Pm4a in common wheat (Triticum aestivum L.) [J].
Hartl, L ;
Mohler, V ;
Zeller, FJ ;
Hsam, SLK ;
Schweizer, G .
GENOME, 1999, 42 (02) :322-329
[8]   DNA RESTRICTION FRAGMENT LENGTH POLYMORPHISMS - A STRATEGY FOR GENETIC-MAPPING OF D GENOME OF WHEAT [J].
KAMMORGAN, LNW ;
GILL, BS ;
MUTHUKRISHNAN, S .
GENOME, 1989, 32 (04) :724-732
[9]   High resolution genetic and physical mapping of molecular markers linked to the Phytophthora resistance gene Rps1-k in soybean [J].
Kasuga, T ;
Salimath, SS ;
Shi, JR ;
Gijzen, M ;
Buzzell, RI ;
Bhattacharyya, MK .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1997, 10 (09) :1035-1044
[10]   A high-density soybean genetic map based on AFLP markers [J].
Keim, P ;
Schupp, JM ;
Travis, SE ;
Clayton, K ;
Zhu, T ;
Shi, LA ;
Ferreira, A ;
Webb, DM .
CROP SCIENCE, 1997, 37 (02) :537-543