Hyperthermia-Mediated Local Drug Delivery by a Bubble-Generating Liposomal System for Tumor-Specific Chemotherapy

被引:172
作者
Chen, Ko-Jie [1 ,2 ]
Chaung, Er-Yuan [1 ,2 ]
Wey, Shiaw-Pyng [3 ,4 ]
Lin, Kun-Ju [3 ,4 ,5 ,6 ]
Cheng, Felice [7 ]
Lin, Chia-Chen [1 ,2 ]
Liu, Hao-Li [8 ]
Tseng, Hsiang-Wen [7 ]
Liu, Chih-Peng [7 ]
Wei, Ming-Cheng [7 ]
Liu, Chun-Min [7 ]
Sung, Hsing-Wen [1 ,2 ]
机构
[1] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 30013, Taiwan
[2] Natl Tsing Hua Univ, Inst Biomed Engn, Hsinchu 30013, Taiwan
[3] Chang Gung Univ, Dept Med Imaging & Radiol Sci, Taoyuan 33302, Taiwan
[4] Chang Gung Univ, Inst Radiol Res, Taoyuan 33302, Taiwan
[5] Chang Gung Mem Hosp, Dept Nucl Med, Taoyuan 33302, Taiwan
[6] Chang Gung Mem Hosp, Mol Imaging Ctr, Taoyuan 33302, Taiwan
[7] Ind Technol Res Inst, Biomed Technol & Device Res Labs, Hsinchu 30013, Taiwan
[8] Chang Gung Univ, Dept Elect Engn, Taoyuan 33302, Taiwan
关键词
thermoresponsive liposome; triggered release; local drug delivery; doxorubicin; biodistribution; TEMPERATURE-SENSITIVE LIPOSOMES; INTENSITY FOCUSED ULTRASOUND; THERMOSENSITIVE LIPOSOME; BREAST-CANCER; THERAPEUTIC-EFFICACY; ANTITUMOR EFFICACY; IN-VITRO; DOXORUBICIN; MODEL; RELEASE;
D O I
10.1021/nn501162x
中图分类号
O6 [化学];
学科分类号
070301 [无机化学];
摘要
As is widely suspected, lysolipid dissociation from liposomes contributes to the intravenous instability of ThermoDox (lysolipid liposomes), thereby impeding its antitumor efficacy. This work evaluates the feasibility of a thermoresponsive bubble-generating liposomal system without lysolipids for tumor-specific chemotherapy. The key component in this liposomal formulation is its encapsulated ammonium bicarbonate (ABC), which is used to actively load doxorubicin (DOX) into liposomes and trigger a drug release when heated locally. Incubating ABC liposomes with whole blood results in a significantly smaller decrease in the retention of encapsulated DOX than that by lysolipid liposomes, indicating superior plasma stability. Biodistribution analysis results indicate that the ABC formulation circulates longer than its lysolipid counterpart. Following the injection of ABC liposome suspension Into mice with tumors heated locally, decomposition of the ABC encapsulated in liposomes facilitates the immediate thermal activation of CO2 bubble generation, subsequently increasing the intratumoral DOX accumulation. Consequently, the antitumor efficacy of the ABC liposomes is superior to that of their lysolipid counterparts. Results of this study demonstrate that this thermoresponsive bubble-generating liposomal system is a highly promising carrier for tumor-specific chemotherapy, especially for local drug delivery mediated at hyperthermic temperatures.
引用
收藏
页码:5105 / 5115
页数:11
相关论文
共 54 条
[1]
A Novel Nanoparticulate Formulation of Arsenic Trioxide with Enhanced Therapeutic Efficacy in a Murine Model of Breast Cancer [J].
Ahn, Richard W. ;
Chen, Feng ;
Chen, Haimei ;
Stern, Stephan T. ;
Clogston, Jeffrey D. ;
Patri, Anil K. ;
Raja, Meera R. ;
Swindell, Elden P. ;
Parimi, Vamsi ;
Cryns, Vincent L. ;
O'Halloran, Thomas V. .
CLINICAL CANCER RESEARCH, 2010, 16 (14) :3607-3617
[2]
Drug delivery systems: Entering the mainstream [J].
Allen, TM ;
Cullis, PR .
SCIENCE, 2004, 303 (5665) :1818-1822
[3]
Ligand-targeted therapeutics in anticancer therapy [J].
Allen, TM .
NATURE REVIEWS CANCER, 2002, 2 (10) :750-763
[4]
Targeted drug delivery to tumors: Myths, reality and possibility [J].
Bae, You Han ;
Park, Kinam .
JOURNAL OF CONTROLLED RELEASE, 2011, 153 (03) :198-205
[5]
The Functional Roles of Poly(Ethylene Glycol)-Lipid and Lysolipid in the Drug Retention and Release from Lysolipid-Containing Thermosensitive Liposomes In Vitro and In Vivo [J].
Banno, Brian ;
Ickenstein, Ludger M. ;
Chiu, Gigi N. C. ;
Bally, Marcel B. ;
Thewalt, Jenifer ;
Brief, Elana ;
Wasan, Ellen K. .
JOURNAL OF PHARMACEUTICAL SCIENCES, 2010, 99 (05) :2295-2308
[6]
Direct 99mTc labeling of pegylated liposomal doxorubicin (Doxil) for pharmacokinetic and non-invasive imaging studies [J].
Bao, AD ;
Goins, B ;
Klipper, R ;
Negrete, G ;
Phillips, WT .
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2004, 308 (02) :419-425
[7]
Doxil® - The first FDA-approved nano-drug: Lessons learned [J].
Barenholz, Yechezkel .
JOURNAL OF CONTROLLED RELEASE, 2012, 160 (02) :117-134
[8]
BARTLETT GR, 1959, J BIOL CHEM, V234, P466
[9]
CO2-"Neutral" Hydrogen Storage Based on Bicarbonates and Formates [J].
Boddien, Albert ;
Gaertner, Felix ;
Federsel, Christopher ;
Sponholz, Peter ;
Mellmann, Doerthe ;
Jackstell, Ralf ;
Junge, Henrik ;
Beller, Matthias .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (28) :6411-6414
[10]
Bolotin Elijah M., 1994, Journal of Liposome Research, V4, P455, DOI 10.3109/08982109409037057