Chemical and Rheological Properties of the, β-Glucan Produced by Pediococcus parvulus 2.6

被引：42

作者：

Velasco, Susana E. ^{[1
,2
]}

Areizaga, Javier ^{[2
]}

Irastorza, Ana ^{[1
]}

Duenas, Maria T. ^{[1
]}

Santamaria, Antxon ^{[2
]}

Munoz, Maria E. ^{[2
]}

机构：

[1] Univ Basque Country, Fac Quim, Dept Quim Aplicada, E-20080 San Sebastian, Spain

[2] Univ Basque Country, Fac Quim, Dept Ciencia & Tecnol Polimeros, E-20080 San Sebastian, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2009年 / 57卷 / 05期

关键词：

Exopolysaccharides; rheological properties; lactic acid bacteria; fermentation; molecular mass; EXOPOLYSACCHARIDE PRODUCTION; LACTOCOCCUS-LACTIS; AQUEOUS-SOLUTIONS; SUGAR METABOLISM; MOLAR-MASS; GROWTH; PEDIOCOCCUS-PARVULUS-2.6; POLYSACCHARIDE; SCLEROGLUCAN; SCATTERING;

D O I：

10.1021/jf803065w

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Some physicochemical and rheological properties of the exopolysaccharide (EPS) produced by Pediococcus parvulus 2.6 were examined. Structural characterization by NMR (H-1 and 2D-COSY) showed that the same EPS, a 2-substituted (1,3)-beta-D-glucan, was synthesized irrespective of sugar source used for growth (glucose, fructose, or maltose). The molecular masses of these beta-glucans were always very high (> 10(6) Da) and influenced by the culture medium or sugar source. The steady shear rheological experiments showed that all concentrations of the P-glucan aqueous solutions exhibited a pseudoplastic behavior at high shear rates. Viscoelastic behavior of P-glucan solutions was determined by dynamic oscillatory analysis. A critical concentration of 0.35% associated with the appearance of entanglements was calculated. The P-glucan adopts an ordered hydrogen bond dependent helical conformation in neutral and slightly alkaline aqueous solutions, which was partly denatured under more alkaline conditions.

引用

页码：1827 / 1834

页数：8

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