Voltammetric sensor for oxidized DNA using ultrathin films of osmium and ruthenium metallopolymers

被引：56

作者：

Mugweru, A

Wang, BQ

Rusling, J

机构：

[1] Univ Connecticut, Dept Chem, Storrs, CT 06269 USA

[2] Univ Connecticut, Ctr Hlth, Dept Pharmacol, Farmington, CT 06032 USA

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 18期

关键词：

D O I：

10.1021/ac049375j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Films containing [Os(bPY)(2)(PVP)(10)Cl](+) and [Ru(bPY)(2)-(PVP)(10)Cl](+) metallopolymers were assembled layer by layer on pyrolytic graphite electrodes to make sensors that selectively detect oxidized DNA. These films showed reversible, independent electrochemistry for electroactive OS3+/OS2+ and Ru3+/Ru2+ centers, with formal potentials of 0.34 and 0.76 Vvs SCE, respectively. The combination of ruthenium and osmium metallopolymers in the films provided a catalytic Os square wave voltammetry (SWV) peak that is mainly selective for 8-oxoguanine and the detection of other oxidized nucleohases from the Ru peak. The method is applicable to measurements on DNA in solution or DNA incorporated into films. Using the Os SWV peak, 1 oxidized nucleobase in 6000 was detected. The sensor is simple and inexpensive, and the approach may be useful for the detection of oxidized DNA as a clinical biomarker for oxidative stress.

引用

页码：5557 / 5563

页数：7

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