Aptamer-Linked Assay for Thrombin Using Gold - Mass Spectrometry Detection

被引:123
作者
Zhao, Qiang [1 ]
Lu, Xiufen [1 ]
Yuan, Chun-Gang [1 ]
Li, Xing-Fang [1 ]
Le, X. Chris [1 ]
机构
[1] Univ Alberta, Dept Lab Med & Pathol, Div Analyt & Environm Toxicol, Edmonton, AB, Canada
关键词
ELECTROCHEMICAL APTASENSOR; AMPLIFICATION; PROTEINS; IMMUNOASSAY; MOLECULES; SELECTION; BIND; NANOPARTICLES; PLATFORM; PEPTIDE;
D O I
10.1021/ac900961y
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We describe a sensitive and specific sandwich assay for human alpha-thrombin. The assay takes advantage of sandwich binding of two affinity aptamers for increased specificity, gold nanoparticles for signal amplification, magnetic beads for fast magnetic separation, and inductively coupled plasma mass spectrometry for ultrasensitive detection. Other proteins, such as immunoglobulin G, serum albumin, transferrin, fibrinogen, and lysozyme did not show interference with the assay for human alpha-thrombin. The detection limit of human alpha-thrombin was as low as 0.5 fmol, corresponding to 10 pM thrombin in 50 mu L, and the dynamic range covered similar to 3 orders of magnitude.
引用
收藏
页码:7484 / 7489
页数:6
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