Aptamer-Linked Assay for Thrombin Using Gold - Mass Spectrometry Detection

被引：123

作者：

Zhao, Qiang ^{[1
]}

Lu, Xiufen ^{[1
]}

Yuan, Chun-Gang ^{[1
]}

Li, Xing-Fang ^{[1
]}

Le, X. Chris ^{[1
]}

机构：

[1] Univ Alberta, Dept Lab Med & Pathol, Div Analyt & Environm Toxicol, Edmonton, AB, Canada

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 17期

关键词：

ELECTROCHEMICAL APTASENSOR; AMPLIFICATION; PROTEINS; IMMUNOASSAY; MOLECULES; SELECTION; BIND; NANOPARTICLES; PLATFORM; PEPTIDE;

D O I：

10.1021/ac900961y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We describe a sensitive and specific sandwich assay for human alpha-thrombin. The assay takes advantage of sandwich binding of two affinity aptamers for increased specificity, gold nanoparticles for signal amplification, magnetic beads for fast magnetic separation, and inductively coupled plasma mass spectrometry for ultrasensitive detection. Other proteins, such as immunoglobulin G, serum albumin, transferrin, fibrinogen, and lysozyme did not show interference with the assay for human alpha-thrombin. The detection limit of human alpha-thrombin was as low as 0.5 fmol, corresponding to 10 pM thrombin in 50 mu L, and the dynamic range covered similar to 3 orders of magnitude.

引用

页码：7484 / 7489

页数：6

共 48 条

[11] Protein detection via direct enzymatic amplification of short DNA aptamers
Fischer, Nicholas O.
Tarasow, Theodore M.
Tok, Jeffrey B. -H.
[J]. ANALYTICAL BIOCHEMISTRY, 2008, 373 (01) : 121 - 128
[12] Methods developed for SELEX
Gopinath, Subash Chandra Bose
[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 387 (01) : 171 - 182
[13] Selection and analytical applications of aptamers
Hamula, Camille L. A.
Guthrie, Jeffrey W.
Zhang, Hongquan
Li, Xing-Fang
Le, X. Chris
[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2006, 25 (07) : 681 - 691
[14] Ultrasensitive electrochemical detection of proteins by amplification of aptamer-nanoparticle bio bar codes
He, Pingli
Shen, Li
Cao, Yunhe
Lia, Defa
[J]. ANALYTICAL CHEMISTRY, 2007, 79 (21) : 8024 - 8029
[15] The bio-barcode assay for the detection of protein and nucleic acid targets using DTT-induced ligand exchange
Hill, Haley D.
Mirkin, Chad A.
[J]. NATURE PROTOCOLS, 2006, 1 (01) : 324 - 336
[16] Imaging and spatial distribution of β-amyloid peptide and metal ions in Alzheimer's plaques by laser ablation-inductively coupled plasma-mass spectrometry
Hutchinson, RW
Cox, AG
McLeod, CW
Marshall, PS
Harper, A
Dawson, EL
Howlett, DR
[J]. ANALYTICAL BIOCHEMISTRY, 2005, 346 (02) : 225 - 233
[17] Hutchinson RW, 2004, CAN J ANAL SCI SPECT, V49, P429
[18] Jayasena SD, 1999, CLIN CHEM, V45, P1628
[19] Integrated nanoparticle-biomolecule hybrid systems: Synthesis, properties, and applications
Katz, E
Willner, I
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (45) : 6042 - 6108
[20] Klussmann S., 2006, APTAMER HDB FUNCTION

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