MicroRNA detection by microarray

被引：371

作者：

Li, Wei ^{[1
]}

Ruan, Kangcheng ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2009年 / 394卷 / 04期

关键词：

MicroRNA; MicroRNA detection; MicroRNA microarray; MicroRNA labeling; SMALL RNAS; RT-PCR; EXPRESSION; REVEALS; IDENTIFICATION; ARRAY; BRAIN; QUANTIFICATION; BIOGENESIS; SIGNATURES;

D O I：

10.1007/s00216-008-2570-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs (miRNAs) are a class of small noncoding RNAs similar to 22 nt in length that regulate gene expression and play fundamental roles in multiple biological processes, including cell differentiation, proliferation and apoptosis as well as disease processes. The study of miRNA has thus become a rapidly emerging field in life science. The detection of miRNA expression is a very important first step in miRNA exploration. Several methodologies, including cloning, northern blotting, real-time RT-PCR, microRNA arrays and ISH (in situ hybridization), have been developed and applied successfully in miRNA profiling. This review discusses the main existing microRNA detection technologies, while emphasizing microRNA arrays.

引用

页码：1117 / 1124

页数：8

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