A standard operating procedure for assessing liquid handler performance in high-throughput screening

被引:34
作者
Taylor, PB
Ashman, S
Baddeley, SM
Bartram, SL
Battle, CD
Bond, BC
Clements, YM
Gaul, NJ
McAllister, WE
Mostacero, JA
Ramon, F
Wilson, JM
Hertzberg, RP
Pope, AJ
Macarron, R
机构
[1] GlaxoSmithKline, Dept Mol Screening, King Of Prussia, PA USA
[2] Dept Assay Methodol Dev, Harlow, Essex, England
[3] Dept Stat Sci, Harlow, Essex, England
[4] Cheminformat, King Of Prussia, PA USA
[5] Cheminformat, Stevenage, Herts, England
[6] Syst Res, King Of Prussia, PA USA
关键词
D O I
10.1177/1087057102238630
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The thrust of early drug discovery in recent years has been toward the configuration of homogeneous miniaturized assays. This has allowed organizations to contain costs in the face of exponential increases in the number of screening assays that need to be run to remain competitive. Miniaturization brings with it an increasing dependence on instrumentation, which over the past several years has seen the development of nanodispensing capability and sophisticated detection strategies. To maintain confidence in the data generated from miniaturized assays, it is critical to ensure that both compounds and reagents have been delivered as expected to the target wells. The authors have developed a standard operating procedure for liquid-handling quality control that has enabled them to evaluate performance on 2 levels. The first level provides for routine daily testing on existing instrumentation, and the second allows for more rigorous testing of new dispensing technologies. The procedure has shown itself to be useful in identifying both method programming and instrumentation performance shortcomings and has provided a means to harmonizing instrumentation usage by assay development and screening groups. The goal is that this type of procedure be used for facilitating the exchange of liquid handler performance data across the industry.
引用
收藏
页码:554 / 569
页数:16
相关论文
共 41 条
[21]   High-throughput screening: new technology for the 21st century [J].
Hertzberg, RP ;
Pope, AJ .
CURRENT OPINION IN CHEMICAL BIOLOGY, 2000, 4 (04) :445-451
[22]   Nanolitre dispensing - a new innovation in robotic liquid handling [J].
James, P ;
Papen, R .
DRUG DISCOVERY TODAY, 1998, 3 (09) :429-430
[23]  
JANZEN B, 1996, J BIOMOL SCREEN, V1, P63
[24]   Test tube's end [J].
Knapp, MR ;
Sundberg, S ;
Parce, JW .
JOURNAL OF BIOMOLECULAR SCREENING, 2000, 5 (01) :9-12
[25]   Beyond the 96-well microplate: Instruments and assay methods for the 384-well format [J].
Kolb, AJ ;
Neumann, K .
JOURNAL OF BIOMOLECULAR SCREENING, 1997, 2 (02) :103-109
[26]   TOWARD A CDNA MAP OF THE HUMAN GENOME [J].
KORENBERG, JR ;
CHEN, XN ;
ADAMS, MD ;
VENTER, JC .
GENOMICS, 1995, 29 (02) :364-370
[27]   Characterization of an inkjet chemical microdispenser for combinatorial library synthesis [J].
Lemmo, AV ;
Fisher, JT ;
Geysen, HM ;
Rose, DJ .
ANALYTICAL CHEMISTRY, 1997, 69 (04) :543-551
[28]  
Lewis R, 1997, SCIENTIST, V11, P18
[29]   Miniaturization of a mammalian cell-based assay: Luciferase reporter gene readout in a 3 microliter 1536-well plate [J].
Maffia, AM ;
Kariv, I ;
Oldenburg, KR .
JOURNAL OF BIOMOLECULAR SCREENING, 1999, 4 (03) :137-142
[30]  
Major J, 1999, J BIOMOL SCREEN, V4, P119, DOI 10.1177/108705719900400304