Detection of oxazolidinone-resistant Enterococcus faecalis and Enterococcus faecium strains by real-time PCR and PCR-restriction fragment length polymorphism analysis

被引：37

作者：

Woodford, N

Tysall, L

Auckland, C

Stockdale, MW

Lawson, AJ

Walker, RA

Livermore, DM

机构：

[1] Cent Publ Hlth Lab, ARMRL, London NW9 5HT, England

[2] Cent Publ Hlth Lab, Lab Enter Pathogens, London NW9 5HT, England

[3] Southampton Publ Hlth Lab, Southampton SO16 6YD, Hants, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 11期

关键词：

D O I：

10.1128/JCM.40.11.4298-4300.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A real-time PCR assay identified linezolid-resistant Enterococcus faecalis and Enterococcus faecium isolates with a G2576U rRNA mutation. PCR-restriction fragment length polymorphism analysis of ribosomal DNA amplicons with NheI also detected this mutation. Both assays detected isolates heterozygous at this position. Recognition of isolates with what is presently the most frequent oxazolidinone resistance mutation may aid surveillance and individual case management.

引用

页码：4298 / 4300

页数：3

共 11 条

[1] Cockerill FR, 2002, ASM NEWS, V68, P77
[2] Novel method for rapid determination of clarithromycin sensitivity in Helicobacter pylori
Gibson, JR
Saunders, NA
Burke, B
Owen, RJ
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (11) : 3746 - 3748
[3] Linezolid-resistant Enterococcus faecium isolated from a patient without prior exposure to an oxazolidinone:: Report from the SENTRY Antimicrobial Surveillance Program
Jones, RN
Della-Latta, P
Lee, LV
Biedenbach, DJ
[J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2002, 42 (02) : 137 - 139
[4] In vitro evaluation of AZD2563, a new oxazolidinone, tested against unusual Gram-positive species
Jones, RN
Biedenbach, DJ
Anderegg, TR
[J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2002, 42 (02) : 119 - 122
[5] Resistance mutations in 23 S rRNA identify the site of action of the protein synthesis inhibitor linezolid in the ribosomal peptidyl transferase center
Kloss, P
Xiong, LQ
Shinabarger, DL
Mankin, AS
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1999, 294 (01) : 93 - 101
[6] Quinupristin/dalfopristin and linezolid: where, when, which and whether to use?
Livermore, DM
[J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2000, 46 (03) : 347 - 350
[7] Resistance to linezolid: Characterization of mutations in rRNA and comparison of their occurrences in vancomycin-resistant enterococci
Prystowsky, J
Siddiqui, F
Chosay, J
Shinabarger, DL
Millichap, J
Peterson, LR
Noskin, GA
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (07) : 2154 - 2156
[8] Rapid identification of methicillin-resistant Staphylococcus aureus from positive blood cultures by real-time fluorescence PCR
Tan, TY
Corden, S
Barnes, R
Cookson, B
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (12) : 4529 - 4531
[9] Linezolid resistance in a clinical isolate of Staphylococcus aureus
Tsiodras, S
Gold, HS
Sakoulas, G
Eliopoulos, GM
Wennersten, C
Venkataraman, L
Moellering, RC
Ferraro, MJ
[J]. LANCET, 2001, 358 (9277) : 207 - 208
[10] Use of a LightCycler gyrA mutation assay for rapid identification of mutations conferring decreased susceptibility to ciprofloxacin in multiresistant Salmonella enterica serotype typhimurium DT104 isolates
Walker, RA
Saunders, N
Lawson, AJ
Lindsay, EA
Dassama, M
Ward, LR
Woodward, MJ
Davies, RH
Liebana, E
Threlfall, EJ
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (04) : 1443 - 1448

← 1 2 →