Safety of ESAT-6

被引：19

作者：

Aggerbeck, Henrik

Madsen, Soren M.

机构：

[1] Statens Serum Inst, Dept Vaccine Dev, DK-2300 Copenhagen S, Denmark

[2] Bioneer AS, DK-2970 Horsholm, Denmark

来源：

TUBERCULOSIS | 2006年 / 86卷 / 05期

关键词：

ESAT-6; safety; tuberculosis; Lactococcus expression;

D O I：

10.1016/j.tube.2005.08.020

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A recombinant dimer of the Mycobacterium tuberculosis (MTb) 6kDa early secreted antigenic target (ESAT-6) was produced in Lactococcus lactis. Pharmacodynamic and safety studies were carried out in guinea pigs, rats, mice and dogs with intradermal (id), subcutaneous (sc) and intravenous (iv) administration of the antigen. In contrast to tuberculin purified protein derivative (PPD) the recombinant dinner (rdESAT-6) was able to discriminate MTb infection from BCG vaccination in vivo. In guinea pigs sensitized by infection with MTb, 1 mu g rcIESAT-6 gave a mean delayed-type hypersensitivity (DTH) response of 22 mm, a significantly stronger reaction than in animals sensitised by the environmental mycobacteria M. kansasii, M. szulgai and M. marinum. rdESAT-6 proved to be a safe tuberculin reagent in a dose range of 1-1000 mu g with no or only minor local reactions. (c) 2005 Elsevier Ltd. All rights reserved.

引用

收藏

页码：363 / 373

页数：11

相关论文

共 41 条

[21] The primary mechanism of attenuation of bacillus Calmette-Guerin is a loss of secreted lytic function required for invasion of lung interstitial tissue [J].

Hsu, T ;

Hingley-Wilson, SM ;

Chen, B ;

Chen, M ;

Dai, AZ ;

Morin, PM ;

Marks, CB ;

Padiyar, J ;

Goulding, C ;

Gingery, M ;

Eisenberg, D ;

Russell, RG ;

Derrick, SC ;

Collins, FM ;

Morris, SL ;

King, CH ;

Jacobs, WR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (21) :12420-12425

[22] THE TUBERCULIN SKIN-TEST [J].

HUEBNER, RE ;

SCHEIN, MF ;

BASS, JB .

CLINICAL INFECTIOUS DISEASES, 1993, 17 (06) :968-975

[23] Molecular characterization of the pH-inducible and growth phase-dependent promoter P170 of Lactococcus lactis [J].

Madsen, SM ;

Arnau, J ;

Vrang, A ;

Givskov, M ;

Israelsen, H .

MOLECULAR MICROBIOLOGY, 1999, 32 (01) :75-87

[24]

MAGNUSSON M, 1958, Bull World Health Organ, V19, P829

[25] Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M-bovis [J].

Mahairas, GG ;

Sabo, PJ ;

Hickey, MJ ;

Singh, DC ;

Stover, CK .

JOURNAL OF BACTERIOLOGY, 1996, 178 (05) :1274-1282

[26] Interpretation of repeated tuberculin tests - Boosting, conversion, and reversion [J].

Menzies, D .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1999, 159 (01) :15-21

[27] The in vitro evolution of BCG vaccines [J].

Mostowy, S ;

Tsolaki, AG ;

Small, PM ;

Behr, MA .

VACCINE, 2003, 21 (27-30) :4270-4274

[28] Human Th1 cell lines recognize the Mycobacterium tuberculosis ESAT-6 antigen and its peptides in association with frequently expressed HLA class II molecules [J].

Mustafa, AS ;

Shaban, FA ;

Al-Attiyah, R ;

Abal, AT ;

El-Shamy, AM ;

Andersen, P ;

Oftung, F .

SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2003, 57 (02) :125-134

[29] Protective effect of a tuberculosis subunit vaccine based on a fusion of antigen 85B and ESAT-6 in the aerosol guinea pig model [J].

Olsen, AW ;

Williams, A ;

Okkels, LM ;

Hatch, G ;

Andersen, P .

INFECTION AND IMMUNITY, 2004, 72 (10) :6148-6150

[30] Specific delayed-type hypersensitivity responses to ESAT-6 identify-tuberculosis-infected cattle [J].

Pollock, JM ;

McNair, J ;

Bassett, H ;

Cassidy, JP ;

Costello, E ;

Aggerbeck, H ;

Rosenkrands, I ;

Andersen, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (05) :1856-1860

← 1 2 3 4 5 →