Secondary ion images of the rodent brain

被引：24

作者：

McCandlish, CA ^{[1
]}

McMahon, JM ^{[1
]}

Todd, PJ ^{[1
]}

机构：

[1] Oak Ridge Natl Lab, Div Chem & Analyt Sci, Oak Ridge, TN 37831 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2000年 / 11卷 / 03期

关键词：

D O I：

10.1016/S1044-0305(99)00145-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sections of biologic tissue obtained from laboratory rodents are prepared and analyzed by secondary ion mass spectrometry. The intensity of phosphocholine secondary ions is used to identify anatomical features of the brain from secondary ion images and to evaluate the effectiveness of procedures developed. Secondary ion emission of phosphocholine (m/z 184), is found to be abundant and its intensity is heterogeneous. Effects of sample thickness are addressed. Correspondence between conventional optical images of stained tissue and secondary ion images shows that successive ion images may be used to produce a three-dimensional map of the brain, i.e., an atlas. (C) 2000 American Society for Mass Spectrometry.

引用

页码：191 / 199

页数：9

共 30 条

[21]

2-N

[22]

ODOM RW, 1993, PRACTICAL SPECTROSC, V16, P345

[23]

PINDER RM, 1973, PROG MED CHEM, V9, P192

[24]

PORCELLATI G, 1985, PHOSPHOLIPIDS NERVOU, P1

[25]

PRAXINOS G, 1986, RAT BRAIN STEREOTAXI

[26] CHARGE COMPENSATION FOR IMAGING LARGE INSULATING SAMPLES BY USING SECONDARY-ION TANDEM MASS-SPECTROMETRY [J].

SHORT, RT ;

MCMAHON, JM ;

HOLLAND, WM ;

TODD, PJ .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (01) :37-43

[27]

SODERBERG M, 1992, J NEUROCHEM, V59, P1646

[28] ORGANIC ION IMAGING USING TANDEM MASS-SPECTROMETRY [J].

TODD, PJ ;

SHORT, RT ;

GRIMM, CC ;

HOLLAND, WM ;

MARKEY, SP .

ANALYTICAL CHEMISTRY, 1992, 64 (17) :1871-1878

[29]

TODD PJ, 1997, ANAL CHEM, V69, P529

[30] RELATIONSHIPS BETWEEN INTERPHASE SCHWANN CELLS AND AXONS BEFORE MYELINATION - QUANTITATIVE ELECTRON-MICROSCOPIC STUDY [J].

WEBSTER, HDF ;

MARTIN, JR ;

OCONNELL, MF .

DEVELOPMENTAL BIOLOGY, 1973, 32 (02) :401-416

← 1 2 3 →