An integrated microfluidic device for large-scale in situ click chemistry screening

被引：81

作者：

Wang, Yanju ^{[3
,4
]}

Lin, Wei-Yu ^{[3
]}

Liu, Kan ^{[3
]}

Lin, Rachel J. ^{[3
]}

Selke, Matthias ^{[4
]}

Kolb, Hartmuth C. ^{[5
]}

Zhang, Nangang ^{[3
,6
]}

Zhao, Xing-Zhong ^{[6
]}

Phelps, Michael E. ^{[3
]}

Shen, Clifton K. F. ^{[3
]}

Faull, Kym F. ^{[1
,2
]}

Tseng, Hsian-Rong ^{[3
]}

机构：

[1] Univ Calif Los Angeles, David Geffen Sch Med, Pasarow Mass Spectrometry Lab, Dept Psychiat & Behav Sci, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Semel Inst Neurosci & Human Behav, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, David Geffen Sch Med, Crump Inst Mol Imaging, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA

[4] Calif State Univ Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90032 USA

[5] Siemens Med Solut USA Inc, Culver City, CA 90230 USA

[6] Wuhan Univ, Sch Phys, Dept Phys, Ctr Nanosci & Nanotechnol, Wuhan 430072, Peoples R China

来源：

LAB ON A CHIP | 2009年 / 9卷 / 16期

关键词：

ORGANIC-SYNTHESIS; COMBINATORIAL LIBRARIES; INHIBITORS; REACTORS; ACETYLCHOLINESTERASE; MICROREACTORS; DISCOVERY; DROPLETS; CHANNELS; SYSTEMS;

D O I：

10.1039/b907430a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An integrated microfluidic device has been developed to perform 1024 in situ click chemistry reactions in parallel using the bovine carbonic anhydrous II (bCAII) click chemistry system as a proof-of-concept study and a rapid hit identification approach using SPE purification and electrospray-ionization mass spectrometry, multiple reaction monitoring (MRM) analysis, all of which improves the sensitivity and throughput of the downstream analysis.

引用

页码：2281 / 2285

页数：5

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