Demonstration of Genetic Exchange During Cyclical Development of Leishmania in the Sand Fly Vector

被引：236

作者：

Akopyants, Natalia S. ^{[2
]}

Kimblin, Nicola ^{[1
]}

Secundino, Nagila ^{[1
]}

Patrick, Rachel ^{[1
]}

Peters, Nathan ^{[1
]}

Lawyer, Phillip ^{[1
]}

Dobson, Deborah E. ^{[2
]}

Beverley, Stephen M. ^{[2
]}

Sacks, David L. ^{[1
]}

机构：

[1] NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA

[2] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA

来源：

SCIENCE | 2009年 / 324卷 / 5924期

关键词：

TRYPANOSOMA-BRUCEI; GENOMIC ORGANIZATION; DONOVANI COMPLEX; MAJOR FRIEDLIN; RECOMBINATION; TRANSMISSION; EXPRESSION; VIRULENCE; MARKER; CROSS;

D O I：

10.1126/science.1169464

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genetic exchange has not been shown to be a mechanism underlying the extensive diversity of Leishmania parasites. We report here evidence that the invertebrate stages of Leishmania are capable of having a sexual cycle consistent with a meiotic process like that described for African trypanosomes. Hybrid progeny were generated that bore full genomic complements from both parents, but kinetoplast DNA maxicircles from one parent. Mating occurred only in the sand fly vector, and hybrids were transmitted to the mammalian host by sand fly bite. Genetic exchange likely contributes to phenotypic diversity in natural populations, and analysis of hybrid progeny will be useful for positional cloning of the genes controlling traits such as virulence, tissue tropism, and drug resistance.

引用

页码：265 / 268

页数：4

共 26 条

[1] Comparisons of mutants lacking the golgi UDP-Galactose or GDP-Mannose transporters establish that phosphoglycans are important for promastigote but not amastigote virulence in Leishmania major [J].

Capul, Althea A. ;

Hickerson, Suzanne ;

Barron, Tamara ;

Turco, Salvatore J. ;

Beverley, Stephen M. .

INFECTION AND IMMUNITY, 2007, 75 (09) :4629-4637

[2] Two functionally divergent UDP-Gal nucleotide sugar transporters participate in phosphoglycan synthesis in Leishmania major [J].

Capul, Althea A. ;

Barron, Tamara ;

Dobson, Deborah E. ;

Turco, Salvatore J. ;

Beverley, Stephen M. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (19) :14006-14017

[3] PLASTICITY IN CHROMOSOME-NUMBER AND TESTING OF ESSENTIAL GENES IN LEISHMANIA BY TARGETING [J].

CRUZ, AK ;

TITUS, R ;

BEVERLEY, SM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (04) :1599-1603

[4] Genomic organization and expression of the expanded SCG/L/R gene family of Leishmania major:: Internal clusters and telomeric localization of SCGs mediating species-specific LPG modifications [J].

Dobson, DE ;

Scholtes, LD ;

Myler, PJ ;

Turco, SJ ;

Beverley, SM .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2006, 146 (02) :231-241

[5] Identification of genes encoding arabinosyltransferases (SCA) mediating developmental modifications of lipophosphoglycan required for sand fly transmission of Leishmania major [J].

Dobson, DE ;

Mengeling, BJ ;

Cilmi, S ;

Hickerson, S ;

Turco, SJ ;

Beverley, SM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (31) :28840-28848

[6] Mechanism of genetic exchange in American trypanosomes [J].

Gaunt, MW ;

Yeo, M ;

Frame, IA ;

Stothard, JR ;

Carrasco, HJ ;

Taylor, MC ;

Mena, SS ;

Veazey, P ;

Miles, GAJ ;

Acosta, N ;

de Arias, AR ;

Miles, MA .

NATURE, 2003, 421 (6926) :936-939

[7] TRISOMY AND CHROMOSOME SIZE CHANGES IN HYBRID TRYPANOSOMES FROM A GENETIC CROSS BETWEEN TRYPANOSOMA-BRUCEI-RHODESIENSE AND TRYPANOSOMA-BRUCEI-BRUCEI [J].

GIBSON, W ;

GARSIDE, L ;

BAILEY, M .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 51 (02) :189-199

[8] Success and virulence in toxoplasma as the result of sexual recombination between two distinct ancestries [J].

Grigg, ME ;

Bonnefoy, S ;

Hehl, AB ;

Suzuki, Y ;

Boothroyd, JC .

SCIENCE, 2001, 294 (5540) :161-165

[9] Sexual reproduction and the evolution of microbial pathogens [J].

Heitman, Joseph .

CURRENT BIOLOGY, 2006, 16 (17) :R711-R725

[10] Analysis of ploidy (in megabase chromosomes) in Trypanosoma brucei after genetic exchange [J].

Hope, M ;

MacLeod, A ;

Leech, V ;

Melville, S ;

Sasse, J ;

Tait, A ;

Turner, CMR .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1999, 104 (01) :1-9

← 1 2 3 →