The nucleotide pool is a significant target for oxidative stress

被引：117

作者：

Haghdoost, Siarnak ^{[1
]}

Sjolander, Lena ^{[1
]}

Czene, Stefan ^{[1
]}

Hanns-Ringdahl, Mats ^{[1
]}

机构：

[1] Stockholm Univ, Dept Genet Microbiol & Toxicol, S-10691 Stockholm, Sweden

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 2006年 / 41卷 / 04期

关键词：

8-oxo-dG; S-OH-dG; nucleotide pool; hMTH1; siRNA; radiation; oxidative stress; free radicals;

D O I：

10.1016/j.freeradbiomed.2006.05.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxidative stress is considered to be one of the most important phenomena involved in the process of aging and age-related diseases. 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) has been frequently used as a marker for oxidative stress. However, the origin of extracellular 8-oxo-dG is not well understood. The aim of this work was to investigate the nucleotide pool and the role of the human muff homologue protein (hMTH1) in the appearance of extracellular 8-oxo-dG in a cellular model system. For this purpose we used primary human fibroblast cells, which were transfected by siRNAs homologous to hMTH1. Extracellular 8-oxo-dG in cell culture media after exposure of the cells to ionizing radiation was measured as enzyme-linked immunosorbent assay reactivity. Our results demonstrate the profound effect of both hMTH1 expression and nucleotide pool size on the cellular excretion of 8-oxo-dG, suggesting that the nucleotide pool is a significant target for the formation of extracellular 8-oxo-dG. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：620 / 626

页数：7

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