Amperometric algal Chlorella vulgaris cell biosensors based on alginate and polypyrrole-alginate gels

被引：50

作者：

Ionescu, Rodica E.

Abu-Rabeah, Khalil

Cosnier, Serge

Durrieu, Claude

Chovelon, Jean-Marc

Marks, Robert S.

机构：

[1] Univ Grenoble 1, CNRS, FR 2607, Inst Chim Mol Grenoble,UMR 5630,Lab Electrochim O, F-38041 Grenoble 9, France

[2] Ben Gurion Univ Negev, Dept Biotechnol Engn, IL-84105 Beer Sheva, Israel

[3] ENTPE, Lab Sci Environm, F-69518 Vaulx En Velin, France

[4] Univ Lyon 1, UMR 5634, Lab Applicat Chim Environm, F-69100 Villeurbanne, France

[5] Natl Inst Biotechnol Negev, IL-84105 Beer Sheva, Israel

来源：

ELECTROANALYSIS | 2006年 / 18卷 / 11期

关键词：

algae; Chlorella vulgaris; alginate; pyrrole-alginate; amperometry;

D O I：

10.1002/elan.200603506

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The successful development and analytical performances of two biosensor configurations based on the entrapment of algal cells of Chlorella vulgaris into either a regular alginate gel or a newly synthesized pyrrole-alginate matrix are reported. These biosensors were compared in terms of their amperometric current measurements to p-nitrophenyl phosphate when used as substrate for the detection of an algal alkaline phosphatase activity. The high stability of the pyrrole-alginate gel when compared to that of the alginate coating is herein demonstrated.

引用

页码：1041 / 1046

页数：6

共 22 条

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