Synthesis of chiral epsilon-lactones in a two-enzyme system of cyclohexanone mono-oxygenase and formate dehydrogenase with integrated bubble-free aeration

被引：77

作者：

Rissom, S

SchwarzLinek, U

Vogel, M

Tishkov, VI

Kragl, U

机构：

[1] FORSCHUNGSZENTRUM JULICH, FORSCHUNGSZENTRUM, INST BIOTECHNOL, D-52425 JULICH, GERMANY

[2] UNIV LEIPZIG, INST ORGAN CHEM, D-04103 LEIPZIG, GERMANY

[3] MOSCOW MV LOMONOSOV STATE UNIV, DEPT CHEM ENZYMOL, MOSCOW 119899, RUSSIA

来源：

TETRAHEDRON-ASYMMETRY | 1997年 / 8卷 / 15期

关键词：

D O I：

10.1016/S0957-4166(97)00311-X

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

A two-enzyme system consisting of a cyclohexanone mono-oxygenase from Acinetobacter NCIMB 9871 and a protein-engineered formate dehydrogenase for the regeneration of the cofactor NADPH was used for the synthesis of chiral epsilon-lactones. 4-Methylcyclohexanone was used as the model substrate yielding (S)-(-)-5-methyl-oxepane-2-one with high chemical and enantiomeric purity. Syntheses were carried out in a repetitive-batch reactor with integrated bubble-free aeration by means of a thin-walled silicon tube. (C) 1997 Elsevier Science Ltd.

引用

页码：2523 / 2526

页数：4

共 20 条

[1] ENZYMATIC BAEYER-VILLIGER TYPE OXIDATIONS OF KETONES CATALYZED BY CYCLOHEXANONE OXYGENASE [J].