Plant sterol-C24-methyl transferases:: Different profiles of tobacco transformed with SMT1 or SMT2

被引:29
作者
Schaeffer, A [1 ]
Bouvier-Navé, P [1 ]
Benveniste, P [1 ]
Schaller, H [1 ]
机构
[1] Univ Strasbourg 1, CNRS, Inst Bot, Inst Biol Mol Plantes,Dept Biosynth & Fonct, F-67083 Strasbourg, France
关键词
D O I
10.1007/s11745-000-0522-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Higher plant cells contain a mixture of 24-desmethyl, 24-methyl(ene), and 24-ethyl(idene) sterols in given proportions according to species but also to cell type. As a first step to investigate the function of such sterol compositions in the physiology of a plant, we have illustrated in the present work the coexistence of two distinct (S)-adenosyl-L-methionine sterol-C24-methyltransferases (SMT) in transgenic Nicotiana tabacum L. Indeed, modulation of the expression of the tobacco gene SMT1-1, which encodes a cycloartenol-C24-methyltransferase, results in variations of the proportion of cycloartenol and a concomitant effect on the proportion of 24-ethyl sterols. Overexpression in tobacco of the Arabidopsis thaliana (L.) Heynh. gene SMT2-1 which encodes a 24-methylene lophenol-C24(1)-methyltransferase, results in a dramatic modification of the ratio of 24-methyl cholesterol to sitosterol associated with a reduced growth, a topic discussed in the present work.
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收藏
页码:263 / 269
页数:7
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