共 23 条
Exogenous Smad3 accelerates wound healing in a rabbit dermal ulcer model
被引:24
作者:
Sumiyoshi, K
Nakao, A
Setoguchi, Y
Okumura, K
Ogawa, H
机构:
[1] Univ Yamanashi, Dept Immunol, Fac Med, Yamanashi 4093898, Japan
[2] Juntendo Univ, Sch Med, Atopy Allergy Res Ctr, Tokyo 113, Japan
[3] Juntendo Univ, Sch Med, Dept Dermatol, Tokyo 113, Japan
[4] Juntendo Univ, Sch Med, Dept Resp Med, Tokyo 113, Japan
关键词:
adenovector;
fibroblast;
Smad7;
D O I:
10.1111/j.0022-202X.2004.22730.x
中图分类号:
R75 [皮肤病学与性病学];
学科分类号:
100206 ;
摘要:
Exogenous administration of transforming growth factor-beta (TGF-beta) improves wound healing by affecting cellular and molecular events involved in tissue repair. But mice with a deficiency of a key TGF-beta signaling intermediate, Smad3, paradoxically showed accelerated cutanenous wound healing, suggesting that endogenous Smad3 had inhibitory effect on cutaneous wound healing. Here we investigated the effect of exogenous expression of Smad3 in dermal fibroblasts on cutaneous wound healing. Subcutaneous injection of adenovirus-containing Smad3 complementary DNA (AdCMV-Smad3) targeting mainly dermal fibroblasts accelerated tissue repair following full-thickness dermal round wounds in rabbit ear as judged by the size of granulation tissue area, number of capillaries, and re-epithelialization rate of the wounds. Expressions of alpha-smooth muscle actin (alpha-SMA), vascular endothelial growth factor (VEGF), and fibroblast growth factor receptor were upregulated in the wounded area injected with AdCMV-Smad3. Consistent with the in vivo findings, overexpression of Smad3 induced alpha-SMA, VEGF, and TGF-beta1 expression and augmented chemotactic response in cultured dermal fibroblasts. Therefore, exogenous administration of Smad3 targeting dermal fibroblasts accelerated tissue repair in a rabbit dermal ulcer model by affecting fibroblast responses associated with wound healing. The results suggest that Smad3, when overexpressed in dermal fibroblasts, can promote wound healing.
引用
收藏
页码:229 / 236
页数:8
相关论文