Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection

被引：58

作者：

Beasley, DWC

Holbrook, MR

da Rosa, APAT

Coffey, L

Carrara, AS

Phillippi-Falkenstein, K

Bohm, RP

Ratterree, MS

Lillibridge, KM

Ludwig, GV

Estrada-Franco, J

Weaver, SC

Tesh, RB

Shope, RE

Barrett, ADT

机构：

[1] UTMB, Dept Pathol, Galveston, TX 77555 USA

[2] Univ Texas, Med Branch, Ctr Biodef & Emerging Infect Dis, Sealy Ctr Vaccine Dev, Galveston, TX 77555 USA

[3] Tulane Univ, Tulane Natl Primate Res Ctr, Covington, LA 70433 USA

[4] Univ Texas, Sch Publ Hlth, Houston, TX 77030 USA

[5] USA, Med Res Inst Infect Dis, Diagnost Syst Div, Ft Detrick, MD 21702 USA

[6] USA, Med Res Inst Infect Dis, Div Virol, Ft Detrick, MD 21702 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 06期

关键词：

D O I：

10.1128/JCM.42.6.2759-2765.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic crossreactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked inummosorbent assays using standardized control antisera and field-collected samples.

引用

页码：2759 / 2765

页数：7

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