Epitope tagging

被引:58
作者
Brizzard, Bill [1 ]
机构
[1] Indiana Univ, Res & Technol Corp, Bloomington, IN 47404 USA
关键词
D O I
10.2144/000112841
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Epitope tagging is widely used in the characterization of newly discovered proteins. This review presents an overview of how the technique evolved and how it is being used today, with a focus on its use in the study of protein-protein interactions. In addition, the evolution of the technique for proteomic analyses is described.
引用
收藏
页码:693 / 695
页数:3
相关论文
共 32 条
[21]   Self-assembling protein microarrays [J].
Ramachandran, N ;
Hainsworth, E ;
Bhullar, B ;
Eisenstein, S ;
Rosen, B ;
Lau, AY ;
Walter, JC ;
LaBaer, J .
SCIENCE, 2004, 305 (5680) :86-90
[22]   Consolidating the set of known human protein-protein interactions in preparation for large-scale mapping of the human interactome [J].
Ramani, AK ;
Bunescu, RC ;
Mooney, RJ ;
Marcotte, EM .
GENOME BIOLOGY, 2005, 6 (05)
[23]   A generic protein purification method for protein complex characterization and proteome exploration [J].
Rigaut, G ;
Shevchenko, A ;
Rutz, B ;
Wilm, M ;
Mann, M ;
Séraphin, B .
NATURE BIOTECHNOLOGY, 1999, 17 (10) :1030-1032
[24]   A multipurpose transposon system for analyzing protein production, localization, and function in Saccharomyces cerevisiae [J].
RossMacdonald, P ;
Sheehan, A ;
Roeder, GS ;
Snyder, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (01) :190-195
[25]   The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins [J].
Schmidt, Thomas G. M. ;
Skerra, Arne .
NATURE PROTOCOLS, 2007, 2 (06) :1528-1535
[26]   Heterodimerization of type II phytochromes in Arabidopsis [J].
Sharrock, RA ;
Clack, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (31) :11500-11505
[27]   SINGLE-STEP PURIFICATION OF POLYPEPTIDES EXPRESSED IN ESCHERICHIA-COLI AS FUSIONS WITH GLUTATHIONE S-TRANSFERASE [J].
SMITH, DB ;
JOHNSON, KS .
GENE, 1988, 67 (01) :31-40
[28]   Epitope tagging genomic DNA using a CD-tagging Tn10 minitransposon [J].
Telmer, CA ;
Berget, PB ;
Ballou, B ;
Murphy, RF ;
Jarvik, JW .
BIOTECHNIQUES, 2002, 32 (02) :422-+
[29]   The green fluorescent protein [J].
Tsien, RY .
ANNUAL REVIEW OF BIOCHEMISTRY, 1998, 67 :509-544
[30]   Purification of a rat neurotensin receptor expressed in Escherichia coli [J].
Tucker, J ;
Grisshammer, R .
BIOCHEMICAL JOURNAL, 1996, 317 :891-899