Immuno-strip biosensor system to detect enrofloxacin residues

The immuno-strip biosensor was developed by combination of immuno-chromatography assay and sandwich enzyme linked immunosorbent assay (ELISA) techniques to detect enrofloxacin, a widely used broad-spectrum antibiotic. A proposed analytical system, consisted of immuno-reaction step and enzyme reaction step, has several advantages such as a very cheap manufacturing cost, a short analysis time (approximately 20 min), an easy-to-use, a high sensitivity, and a qualitatively confirmation by bare eyes. Enzyme reaction step in a proposed analytical tool could enhance the signal of immunosensor, resulting in the high sensitivity. A proposed system was successfully applied to detect enrofloxacin in a range of 100-10000 ppb. The lower detection limit was 100 ppb, which is the maximum residue limit of enrofloxacin in animal product. (C) 2009 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.