Signature-tagged mutagenesis: barcoding mutants for genome-wide screens

被引：87

作者：

Mazurkiewicz, Piotr

Tang, Christoph M.

Boone, Charles

Holden, David W.

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Infect Dis, Ctr Mol Microbiol & Infect, London SW7 2AZ, England

[2] Univ Toronto, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada

来源：

NATURE REVIEWS GENETICS | 2006年 / 7卷 / 12期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

D O I：

10.1038/nrg1984

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

DNA signature tags (molecular barcodes) facilitate functional screens by identifying mutants in mixed populations that have a reduced or increased adaptation to a particular environment. Many innovative adaptations and refinements in the technology have been described since its original use with Salmonella; they have yielded a wealth of information on a broad range of biological processes - mainly in bacteria, but also in yeast and other fungi, viruses, parasites and, most recently, in mammalian cells. By combining whole-genome microarrays and comprehensive ordered libraries of mutants, high-throughput functional screens can now be achieved on a genomic scale.

引用

页码：929 / 939

页数：11

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