Overexpression of hsa-miR-939 follows by NGFR down-regulation and apoptosis reduction

被引：11

作者：

Aghdaei, Fahimeh Hosseini ^{[1
]}

Soltani, Bahram M. ^{[1
]}

Dokanehiifard, Sadat ^{[1
]}

Mowla, Seyed Javad ^{[1
]}

Soleimani, Masoud ^{[2
]}

机构：

[1] Tarbiat Modares Univ, Fac Biol Sci, Dept Mol Genet, Tehran, Iran

[2] Tarbiat Modares Univ, Dept Hematol, Fac Med Sci, Tehran, Iran

来源：

JOURNAL OF BIOSCIENCES | 2017年 / 42卷 / 01期

关键词：

Apoptosis; hsa-miR-939; luciferase assay; neurotrophin signalling; NGFR; NERVE GROWTH-FACTOR; P75 NEUROTROPHIN RECEPTOR; CELL LINES; P75(NTR); EXPRESSION; INDUCTION; NEURONS; TARGET; BRAIN; DEATH;

D O I：

10.1007/s12038-017-9669-6

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

Neurotrophin receptors play a crucial role in neuronal survival, differentiation and regeneration. Nerve growth factor receptor (NGFR) or P75(NTR) is a neurotrophin receptor that is involved in many pathological conditions including cancers. Genetic factors that are involved in regulation of neurotrophin receptors are under intense investigation. MiRNAs are novel regulators of signalling pathways that are candidates for regulation of neurotrophin receptors. Computational programs predicted that NGFR gene is a bona fide target for hsa-miR-939. RT-qPCR, Western analysis and dual luciferase assay evidences indicated that NGFR transcript is targeted by hsa-miR-939. Also, hsa-miR-939 overexpression brought about down-regulation of NGFR expression in U87 cell line, followed by cell death rate reduction, detected by flow cytometry. Taken together, here for the first time, hsa-miR-939 is introduced as a novel key regulator of NGFR expression and its involvement in cell death/survival processes is suggested.

引用

页码：23 / 30

页数：8

共 43 条

[21]

The p75 neurotrophin receptor is a central regulator of glioma invasion [J].