Spectrum-effect relationships between UPLC fingerprints and bioactivities of crude secondary roots of Aconitum carmichaelii Debeaux (Fuzi) and its three processed products on mitochondrial growth coupled with canonical correlation analysis

被引：96

作者：

Zheng, Quanfu ^{[1
,2
]}

Zhao, Yanling ^{[2
]}

Wang, Jiabo ^{[2
]}

Liu, Tiantian ^{[1
,2
]}

Zhang, Bo ^{[5
]}

Gong, Man ^{[3
]}

Li, Jianyu ^{[3
]}

Liu, Honghong ^{[3
]}

Han, Bin ^{[4
]}

Zhang, Yaming ^{[2
]}

Song, Xueai ^{[3
]}

Li, Yonggang ^{[3
]}

Xiao, Xiaohe ^{[2
]}

机构：

[1] Chengdu Univ Tradit Chinese Med, Coll Pharm, Chengdu 611137, Peoples R China

[2] 302 Hosp Peoples Liberat Army, China Mil Inst Chinese Med, Beijing 100039, Peoples R China

[3] 302 Hosp Peoples Liberat Army, Dept Integrat Med Ctr, Beijing 100039, Peoples R China

[4] Guang Dong Pharm Coll, Dept Pharm, Guangzhou 51006, Guangdong, Peoples R China

[5] Harbin Med Univ, Hosp Affiliated 2, Harbin 150086, Heilongjiang Pr, Peoples R China

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2014年 / 153卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Crude secondary roots of Aconitum carmichaelii Debeaux (Fuzi); Processed products; Mitochondria; UPLC fingerprints; Microcalorimetry; Spectrum-effect relationships; CHROMATOGRAPHIC FINGERPRINT; ISOTHERMAL MICROCALORIMETRY; ESCHERICHIA-COLI;

D O I：

10.1016/j.jep.2014.03.011

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Ethnopharmacological relevance: The crude secondary roots of Aconitum carmichaelii Debeaux (Fuzi), together with its processed products, including Yanfuzi, Heishunpian and Paofupian, are commonly applied in clinic using for thousands of years, such as collapse, syncope, rheumatic fever, painful joints and various tumors. Aim of the study: To explore the different effects of Fuzi and its processed products on energy metabolism, with mitochondria as the model with the aim of guiding the clinical use of Fuzi and its products. fingerprints of Fuzi, Yanfuzi, Heishunpian and Paofupian were established by Ultra-high Performance Liquid Chromatography (UPLC) and effects of Fuzi and its processed products on rat's liver's mitochondrial metabolism were studied by microcalorimetry. Spectrum-effect relationships between UPLC fingerprints and energy metabolism of mitochondria were investigated using canonical correlation analysis (CCA). Results: Because of their inherent differences in chemical compositions, the main activities of energy metabolism of mitochondria were different among Fuzi and its processed products. The potential bioactivity sequence of the tested products was Fuzi > Heishunpian > Paofupian > Yanfuzi. Results of CCA showed that compounds mesaconitine, benzoylaconitine, and benzoylhypacoitine might be the principal active components. Conclusion: Altogether, this work provides a general model of combination of UPLC and microcalorimetry to study the spectrum-effect relationships of Fuzi and its processed products which can offer some references for detecting principal components of traditional Chinese medicine on bioactivity to mitochondrial growth. (c) 2014 Elsevier Ireland Ltd. All rights reserved.

引用

页码：615 / 623

页数：9

共 35 条

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